ADIPOR1 ELISA Kit (Human) (OKCD06074)

Catalog No: OKCD06074

Size:96WELLS

ADIPOR1 ELISA Kit (Human) (OKCD06074) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Adiponectin Receptor 1 (ADIPOR1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Adiponectin Receptor 1 (ADIPOR1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Adiponectin Receptor 1 (ADIPOR1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Adiponectin Receptor 1 (ADIPOR1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.78-50ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Adiponectin Receptor 1 (ADIPOR1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Adiponectin Receptor 1 (ADIPOR1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • ADIPOR1 Microplate: 96 Wells (12 x 8 Well strips)
  • ADIPOR1 Lyophilized Standard: 2
  • 100X ADIPOR1 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 2 mL
  • Stop Solution: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 0.27ng/mL

Target Info

• Gene Symbol: ADIPOR1
• Gene Full Name: adiponectin receptor 1
• Alias Symbols: ACDCR1;adiponectin receptor protein 1;CGI45;CGI-45;PAQR1;progestin and adipoQ receptor family member 1;progestin and adipoQ receptor family member I;TESBP1A.
• NCBI Gene Id: 51094
• Protein Name: Adiponectin receptor protein 1
• Description of Target: Receptor for ADIPOQ, an essential hormone secreted by adipocytes that regulates glucose and lipid metabolism (PubMed:25855295, PubMed:12802337). Required for normal glucose and fat homeostasis and for maintaining a normal body weight. ADIPOQ-binding activates a signaling cascade that leads to increased AMPK activity, and ultimately to increased fatty acid oxidation, increased glucose uptake and decreased gluconeogenesis. Has high affinity for globular adiponectin and low affinity for full-length adiponectin (By similarity).
• Uniprot ID: Q96A54
• Protein Accession #: NP_001277482.1
• Nucleotide Accession #: NM_001290553.1