ADIPOQ Recombinant Protein (Human) (OPED00006)

Catalog No: OPED00006

Size:50UG

Figure: Superdex 200 analysis of Adiponectin (human) (recombinant) produced in mammalian cells (HEK 293). The different forms of adiponectin are indicated by arrows: High molecular weight (HMW; representing 12-18-mer species) and hexamer. The red arrow indicates where should elute the trimeric state of adiponectin. For further information on adiponectin forms, see Tsao et al., 2002 and Pajvani et al., 2003, in General Literature References.

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Product Format: Lyophilized. Contains PBS.
• Host: HEK 293 cells
• Additional Information: Historical results have shown that adiponectin activates AMPK.
  FLAG is a registered trademark of Sigma-Aldrich Co.
• Reconstitution and Storage: Reconstitute with 50 ul sterile distilled water. Further dilutions should be made with medium containing 5% fetal calf serum or other carrier protein. Store at -20C
• Purity: >90% (SDS-PAGE)
• Reference: Activation of the LH receptor up regulates the type 2 adiponectin receptor in human granulosa cells: E.P. 3rd Wickham, et al.; J. Assist. Reprod. Genet. 30, 963 (2013), Abstract; Full Text
  Adiponectin and its receptors in the ovary: further evidence for a link between obesity and hyperandrogenism in polycystic ovary syndrome: F.V. Comim, et al.; PLoS One 8, e80416 (2013), Abstract; Full Text
  Adiponectin inhibits neutrophil phagocytosis of Escherichia coli by inhibition of PKB and ERK 1/2 MAPK signalling and Mac-1 activation: A. Rossi, et al.; PLoS One 8, e69108 (2013), Abstract; Full Text
  Adiponectin induces CCL20 expression synergistically with IL-6 and TNF-alpha in THP-1 macrophages: M. Suzuki, et al.; Cytokine 58, 344 (2012), Abstract;
  Inhibition of smooth muscle cell proliferation by adiponectin requires proteolytic conversion to its globular form: M. Fuerst, et al.; J. Endocrinol. 215, 107 (2012), Abstract; Full Text
  Globular adiponectin counteracts VCAM-1-mediated monocyte adhesion via AdipoR1/NF-kappa B/COX-2 signaling in human aortic endothelial cells: F. Addabbo, et al.; Am. J. Physiol. Endocrinol. Metab. 301, E1143 (2011), Application(s): WB of human aortic endothelial cells, Abstract; Full Text
  Adipose tissue explants and MDCK cells reciprocally regulate their morphogenesis in coculture: K. Udo, et al.; Kidney Int. 78, 60 (2010), Abstract;
  Sialic acid modification of adiponectin is not required for multimerization or secretion but determines half-life in circulation: A.A. Richards, et al.; Mol. Endocrinol. 24, 229 (2010), Abstract;
  Leptin stimulates fatty acid oxidation and peroxisome proliferator-activated receptor alpha gene expression in mouse C2C12 myoblasts by changing the subcellular localization of the alpha 2 form of AMP-activated protein kinase: A. Suzuki, et al.; Mol. Cell. Biol. 27, 4317 (2007), Abstract;

Target Info

• Gene Symbol: ADIPOQ
• Gene Full Name: adiponectin, C1Q and collagen domain containing
• Alias Symbols: 30 kDa adipocyte complement-related protein;ACDC;ACRP30;adipocyte complement-related 30 kDa protein;Adipocyte, C1q and collagen domain-containing protein;adiponectin;adipose most abundant gene transcript 1 protein;adipose specific collagen-like factor;ADIPQTL1;ADPN;APM1;APM-1;GBP28;Gelatin-binding protein;gelatin-binding protein 28.
• NCBI Gene Id: 9370
• Protein Name: Adiponectin
• Description of Target: Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW.
• Uniprot ID: Q15848
• Protein Accession #: NP_001214.1
• Nucleotide Accession #: NM_001223.4
• Protein Size (# AA): Recombinant
• Molecular Weight: 33 kDa