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Catalog No: OKCD01799 (Formerly GWB-KBBBR4)
Size:96WELLS
Price: $675.00
SKU
OKCD01799
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Datasheets/ManualsPrintable datasheet for ADIG ELISA kit (Human)
Product Info
Predicted Species ReactivityHuman
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColormetric|Regular detection
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)94-105%79-89%80-89%93-102%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Adipogenin (ADIG). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Adipogenin (ADIG). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Adipogenin (ADIG), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Adipogenin (ADIG) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range1.56-100ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum88-9592
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Adipogenin (ADIG) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Adipogenin (ADIG) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-ADIG Microplate96 Wells (12 x 8 Well Strips)
ADIG Lyophilized Standard2 x 100 ng
100X Biotinylated ADIG Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C, -20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity< 0.56 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Adipogenin (ADIG). No significant cross-reactivity or interference between Adipogenin (ADIG) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolADIG
Gene Full NameAdipogenin
Alias SymbolsSMAF1
NCBI Gene Id149685
Protein NameAdipogenin
Description of TargetPlays a role in stimulating adipocyte differentiation and development.
Uniprot IDQ0VDE8
Protein Accession #NP_001018092.1
Nucleotide Accession #NM_001018082.2
Protein Size (# AA)80
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