Catalog No: OKEH02633
Size:96T
Price: $525.00
SKU
OKEH02633
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ADH1A ELISA Kit (Human) : 96 Wells (OKEH02633)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology ADH1A ELISA Kit (Human) (OKEH02633) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Human ADH1A antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated ADH1A and incubated. The ADH1A found in the sample or standards competes with the biotinylated ADH1A for limited binding sites on the immobilized anti-Human ADH1A antibody. Excess unbound biotinylated ADH1A and sample or standard ADH1A is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated ADH1A captured in the well and inversely proportional to the amount of ADH1A which was contained in the sample or standard.
ELISA Kit Range0.312-20ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 99%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 3.7% (n = 20)
Mean Inter-assay CV%: < 7.9% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-ADH1A Microplate96 Wells (12 x 8 Well strips)
ADH1A Lyophilized Standard2
Sample Diluent1 x 20 mL
100X ADH1A-Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Urine, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity0.1 ng/mL
SpecificityNatural and recombinant Human Alcohol dehydrogenase 1A
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolADH1A
Gene Full Namealcohol dehydrogenase 1A (class I), alpha polypeptide
Alias SymbolsADH, alpha subunit;ADH1;alcohol dehydrogenase 1 (class I), alpha polypeptide;alcohol dehydrogenase 1A;alcohol dehydrogenase subunit alpha;aldehyde reductase.
NCBI Gene Id124
Protein NameP07327
Description of TargetAlcohol dehydrogenase 1A
Uniprot IDhttps://www.uniprot.org/uniprot/P07327
Protein Accession #NP_000658.1
Nucleotide Accession #NM_000667.3
Protein Size (# AA)375
Molecular Weight40 kDa
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