Adgre1 ELISA Kit (Mouse) (OKEH04581)

Catalog No: OKEH04581

Size:96T

Mouse Adgre1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse, Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~3 Hours
• ELISA Kit Principle: Aviva Systems Biology Adgre1 ELISA Kit (Mouse) (OKEH04581) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Adgre1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Adgre1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Adgre1 captured in well.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Mean Intra-Assay CV: < 10%
  Mean Inter-Assay CV: < 12%
• ELISA Kit Component:
  • Anti-Adgre1 Microplate: 96 Wells (12 x 8 Well Strips)
  • Adgre1 Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • Adgre1 Biotinylated Detector Antibody: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • HRP Avidin Conjugate: 1 x 120 uL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.091 ng/mL
• Specificity: Natural and recombinant Mouse EGF-like module-containing mucin-like hormone receptor-like 1
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Adgre1
• Gene Full Name: adhesion G protein-coupled receptor E1
• Alias Symbols: adhesion G protein-coupled receptor E1;cell surface glycoprotein F4/80;DD7A5-;DD7A5-7;EGF-like module containing, mucin-like, hormone receptor-like sequence 1;EGF-like module receptor 1;EGF-like module-containing mucin-like hormone receptor-like 1;EGF-TM7;Emr;Emr1;EMR1 hormone receptor;F4/80;Gpf480;Ly7;Ly71;TM7L;TM7LN3.
• NCBI Gene Id: 13733
• Protein Name: Q61549
• Description of Target: Adhesion G protein-coupled receptor E1
• Uniprot ID: https://www.uniprot.org/uniprot/Q61549
• Protein Accession #: NP_034260.1
• Nucleotide Accession #: NM_010130.4
• Protein Size (# AA): 931
• Molecular Weight: 102 KDa