ADD3 Antibody - middle region (OASG00216)

Catalog No: OASG00216

Size:100 uL

Western Blot using 3T3

Western Blot using HepG2

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Liquid. 1 mg/mL in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: WB, IHC, IF
• Reconstitution and Storage: Store at -20C. Avoid repeated freeze/thaw cycles.
• Immunogen: Synthesized peptide derived from the Internal region of human Adducin Gamma. Location: 400-480aa.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Concentration: 1 mg/ml
• Specificity: Adducin Gamma Polyclonal Antibody detects endogenous levels of Adducin Gamma protein.
• Application Info: WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:10000;

Target Info

• Gene Symbol: ADD3
• Gene Full Name: adducin 3 (gamma)
• Alias Symbols: ADDL, CPSQ3
• NCBI Gene Id: 120; 27360; 25230
• Description of Target: Adducins are heteromeric proteins composed of different subunits referred to as adducin alpha, beta and gamma. The three subunits are encoded by distinct genes and belong to a family of membrane skeletal proteins involved in the assembly of spectrin-actin network in erythrocytes and at sites of cell-cell contact in epithelial tissues. While adducins alpha and gamma are ubiquitously expressed, the expression of adducin beta is restricted to brain and hematopoietic tissues. Adducin, originally purified from human erythrocytes, was found to be a heterodimer of adducins alpha and beta. Polymorphisms resulting in amino acid substitutions in these two subunits have been associated with the regulation of blood pressure in an animal model of hypertension. Heterodimers consisting of alpha and gamma subunits have also been described. Structurally, each subunit is comprised of two distinct domains. The amino-terminal region is protease resistant and globular in shape, while the carboxy-terminal region is protease sensitive. The latter contains multiple phosphorylation sites for protein kinase C, the binding site for calmodulin, and is required for association with spectrin and actin. Alternatively spliced adducin gamma transcripts encoding different isoforms have been described. The functions of the different isoforms are not known.
• Uniprot ID: Q9UEY8, Q9QYB5, Q62847
• Molecular Weight: 79 kDa