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Catalog No: OKCD07344
Size:96WELLS
Price: $650.00
SKU
OKCD07344
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Datasheets/ManualsPrintable datasheet for ADCYAP1 ELISA Kit (Mouse) (OKCD07344)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pituitary Adenylate Cyclase Activating Peptide (PACAP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Pituitary Adenylate Cyclase Activating Peptide (PACAP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pituitary Adenylate Cyclase Activating Peptide (PACAP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Pituitary Adenylate Cyclase Activating Peptide (PACAP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range7.8-500pg/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Pituitary Adenylate Cyclase Activating Peptide (PACAP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Pituitary Adenylate Cyclase Activating Peptide (PACAP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
ADCYAP1 Microplate96 Wells (12 x 8 Well strips)
ADCYAP1 Lyophilized Standard2
100X ADCYAP1 HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 2 mL
Stop Solution1 x 20 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Sensitivity< 3.2pg/mL
Gene SymbolAdcyap1
Gene Full Nameadenylate cyclase activating polypeptide 1
Alias SymbolsP;PACAP;pituitary adenylate cyclase-activating polypeptide.
NCBI Gene Id11516
Protein NamePituitary adenylate cyclase-activating polypeptide
Description of TargetBinding to its receptor activates G proteins and stimulates adenylate cyclase in pituitary cells (By similarity). Promotes neuron projection development through the RAPGEF2/Rap1/B-Raf/ERK pathway (By similarity). In chromaffin cells, induces long-lasting increase of intracellular calcium concentrations and neuroendocrine secretion (By similarity). Involved in the control of glucose homeostasis, induces insulin secretion by pancreatic beta cells (PubMed:23913443).
Uniprot IDO70176
Protein Accession #NP_001302432.1
Nucleotide Accession #NM_001315503.1
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