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Catalog No: OKEH04828
Size:96T
Price: $525.00
SKU
OKEH04828
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
COA DatasheetPrintable COA datasheet for OKEH04828
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~3 Hours
ELISA Kit PrincipleAviva Systems Biology ADCYAP1 ELISA Kit (Human) (OKEH04828) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ADCYAP1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ADCYAP1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ADCYAP1 captured in well.
ELISA Kit Range7.8-500pg/mL
ELISA Kit ReproducibilityMean Intra-Assay CV: < 10%
Mean Inter-Assay CV: < 12%
ELISA Kit Component
ComponentAmount
ADCYAP1 Microplate96 Wells (12 x 8 Well Strips)
ADCYAP1 Standard2
Sample Diluent1 x 20 mL
ADCYAP1 Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Sensitivity2.1 pg/mL
SpecificityNatural and recombinant Human Pituitary adenylate cyclase-activating polypeptide
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolADCYAP1
Gene Full Nameadenylate cyclase activating polypeptide 1
Alias Symbolsadenylate cyclase activating polypeptide 1 (pituitary);PACAP;pituitary adenylate cyclase-activating polypeptide;prepro-PACAP.
NCBI Gene Id116
Protein NameP18509
Description of TargetPituitary adenylate cyclase-activating polypeptide
Uniprot IDhttps://www.uniprot.org/uniprot/P18509
Protein Accession #NP_001093203.1
Nucleotide Accession #NM_001099733.1
Protein Size (# AA)176
Molecular Weight19 kDa
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