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Catalog No: OKCD00836 (Formerly GWB-KBBFJ9)
Size:96WELLS
Price: $650.00
SKU
OKCD00836
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Datasheets/ManualsPrintable datasheet for ADCY6 ELISA Kit (Human) (OKCD00836)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
88-102%82-103%91-105%79-101%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Adenylate Cyclase 6 (ADCY6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Adenylate Cyclase 6 (ADCY6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Adenylate Cyclase 6 (ADCY6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Adenylate Cyclase 6 (ADCY6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.312-20ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
89-10394
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Adenylate Cyclase 6 (ADCY6) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Adenylate Cyclase 6 (ADCY6) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-ADCY6 Microplate96 Wells (12 x 8 Well Strips)
ADCY6 Lyophilized Standard2 x 20 ng
100X Biotinylated ADCY6 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity< 0.128 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Adenylate Cyclase 6 (ADCY6). No significant cross-reactivity or interference between Adenylate Cyclase 6 (ADCY6) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolADCY6
Gene Full Nameadenylate cyclase 6
Alias SymbolsAC6;adenylate cyclase type 6;adenylate cyclase type VI;Adenylyl cyclase 6;ATP pyrophosphate-lyase 6;ca(2+)-inhibitable adenylyl cyclase;LCCS8.
NCBI Gene Id112
Protein NameAdenylate cyclase type 6
Description of TargetCatalyzes the formation of the signaling molecule cAMP downstream of G protein-coupled receptors (PubMed:17916776, PubMed:17110384). Functions in signaling cascades downstream of beta-adrenergic receptors in the heart and in vascular smooth muscle cells (PubMed:17916776). Functions in signaling cascades downstream of the vasopressin receptor in the kidney and has a role in renal water reabsorption. Functions in signaling cascades downstream of PTH1R and plays a role in regulating renal phosphate excretion. Functions in signaling cascades downstream of the VIP and SCT receptors in pancreas and contributes to the regulation of pancreatic amylase and fluid secretion (By similarity). Signaling mediates cAMP-dependent activation of protein kinase PKA. This promotes increased phosphorylation of various proteins, including AKT. Plays a role in regulating cardiac sarcoplasmic reticulum Ca(2+) uptake and storage, and is required for normal heart ventricular contractibility. May contribute to normal heart function (By similarity). Mediates vasodilatation after activation of beta-adrenergic receptors by isoproterenol (PubMed:17916776). Contributes to bone cell responses to mechanical stimuli (By similarity).
Uniprot IDO43306
Protein Accession #NP_056085
Nucleotide Accession #NM_015270.4
Protein Size (# AA)1168
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