MMP3 ELISA Kit (Dog) (OKCA02056)

Catalog No: OKCA02056

Size:96 Wells

Dog MMP3 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Dog
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Sample Type	Dilution	Mean (%)	Range (%)
  Serum (n=4)	Neat	92	88-96
  Serum (n=4)	1:2	89	85-96
  Serum (n=4)	1:4	93	89-97
  Serum (n=4)	1:8	99	92-105

• ELISA Kit Principle: Aviva Systems Biology MMP3 ELISA Kit (Dog) (OKCA02056) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MMP3 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MMP3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample MMP3 captured in well.
• ELISA Kit Range: 1.56 ng/mL-100 ng/mL
• ELISA Kit Recovery:

  Sample Type	Mean (%)	Range (%)
  Serum (n=5)	95	91-99
  EDTA plasma (n=4)	92	87-96

• ELISA Kit Reproducibility: Mean Intra-Assay Variation <10% CV (n=20)
  Mean Inter-Assay Variation <12% CV (n=8)
• ELISA Kit Component:
  • Anti-MMP3 Microplate: 96 Wells (12 x 8 Well strips)
  • MMP3 Lyophilized Standard: 2 x 100 ng
  • 100X Biotinylated MMP3 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Cell Culture Supernates, Urine, Tissue Homogenates
• Sensitivity: 0.39 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: MMP3
• Alias Symbols: Matrix metalloproteinase-3, MMP-3, SL-1, Stromelysin-1
• NCBI Gene Id: 403445
• Protein Name: Stromelysin-1
• Description of Target: Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase.
• Uniprot ID: Q6Y4Q5
• Protein Accession #: NP_001002967
• Nucleotide Accession #: NM_001002967