Catalog No: OKEH03453
Size:96T
Price: $525.00
SKU
OKEH03453
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology ADAMTS5 ELISA Kit (Mouse) (OKEH03453) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Adamts5 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Adamts5 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Adamts5 captured in well.
ELISA Kit Range15.6-1000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 108%
ELISA Kit ReproducibilityMean Intra-assay CV%: <= 6.2% (n = 20)
Mean Inter-assay CV%: <= 7.7% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-Adamts5 Microplate96 Wells (12 x 8 Well strips)
Adamts5 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated Adamts5 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity7.98 pg/mL
SpecificityNatural and recombinant Mouse A disintegrin and metalloproteinase with thrombospondin motifs 5
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolAdamts5
Gene Full Namea disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 5 (aggrecanase-2)
Alias Symbols9530092O11Rik;A disintegrin and metalloproteinase with thrombospondin motifs 5;ADAM-;ADAMTS1;ADAMTS11;ADAM-TS5;ADMP-2;aggrecanase-2;AI481094;ASMP-2;implantin.
NCBI Gene Id23794
Protein NameQ9R001
Description of TargetA disintegrin and metalloproteinase with thrombospondin motifs 5
Uniprot IDhttps://www.uniprot.org/uniprot/Q9R001
Protein Accession #NP_035912.2
Nucleotide Accession #NM_011782.2
Protein Size (# AA)930
Molecular Weight102 kDa
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