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Catalog No: OKBB00627
Size:96 Wells
Price: $583.00
SKU
OKBB00627
Availability: Domestic: within 1-2 week delivery | International: within 1-2 week delivery
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ApplicationELISA
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration2.5 Hours
ELISA Kit PrincipleAviva's human ADAMTS4 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for ADAMTS4 has been precoated onto 96-well plates. Standards(CHO, F213-C685) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ADAMTS4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human ADAMTS4 amount of sample captured in plate.
ELISA Kit Range625 pg/ml - 40,000 pg/ml
ELISA Kit Reproducibility
Intra-Assay PrecisionInter-Assay Precision
Sample123123
n 161616242424
Mean (pg/mL)5.912216.51423
SD0.2480.6121.20.3770.8821.66
CV%4.25.15.75.86.37.2
ELISA Kit Component
ComponentAmount
96-Well plate Pre-coated with Anti-Human ADAMTS4 Antibody1 x (12 x 8 Well Strip)
Lyophilized Recombinant Human ADAMTS4 Standard2 x 40 ng/tube
100X Biotinylated Anti-Human ADAMTS4 Antibody1 x 130 uL
100X Avidin-Biotin-Peroxidase Complex (ABC)1 x 130 uL
Sample Diluent Buffer1 x 30 mL
Antibody Diluent Buffer1 x 12 mL
ABC Diluent Buffer1 x 12 mL
TMB Color Developing Agent1 x 10 mL
TMB Stop Solution1 x 10 mL
10X Wash Buffer1 x 30 mL
Reconstitution and StorageStore at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Sample TypeCell culture supernatants, serum and plasma (heparin, EDTA)
Sensitivity<20 pg/ml
SpecificityNatural and recombinant human ADAMTS4
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
ImmunogenExpression system for standard: CHO; Immunogen sequence: F213-C685
DescriptionFor quantitative detection of human ADAMTS4 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Gene SymbolADAMTS4
Gene Full NameADAM metallopeptidase with thrombospondin type 1 motif, 4
Alias SymbolsA disintegrin and metalloproteinase with thrombospondin motifs 4, A disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif 4, A disintegrin like and metalloprotease with thrombospondin type 1 motif 4, ADAM metallopeptidase with thrombospondin type 1 motif 4, ADAM TS 4, ADAM TS4, ADAM-TS 4, ADAM-TS4, ADAMTS 2, ADAMTS 4, ADAMTS 4, ADAMTS-4, ADAMTS2, ADAMTS4, ADAMTS4 protein, ADMP 1, ADMP-1, ADMP1, Aggrecanase 1, Aggrecanase 1, Aggrecanase-1, Aggrecanase1, ATS4_HUMAN, KIAA0688, KIAA0688
NCBI Gene Id9507
Protein NameA disintegrin and metalloproteinase with thrombospondin motifs 4
Description of TargetADAMTS4, A disintegrin and metalloproteinase with thrombospondin motifs 4 is an enzyme that in humans is encoded by the ADAMTS4 gene. ADAMTS4 is a member of the large ADAMTS family of zinc-dependent proteases. The human ADAMTS4 gene is mapped to chromosome 1 by somatic cell hybrid analysis. The enzyme encoded by this gene lacks a C-terminal TS motif. It is responsible for the degradation of aggrecan, a major proteoglycan of cartilage, and brevican, a brain-specific extracellular matrix protein. The cleavage of aggrecan and brevican suggests key roles of this enzyme in arthritic disease and in the central nervous system, potentially, in the progression of glioma.
Uniprot IDO75173
Protein Accession #NP_005090.3
Nucleotide Accession #NM_005099.4
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