ADAMTS2 ELISA Kit (Bovine) (OKEH08477)

Catalog No: OKEH08477

Size:96T

Bovine ADAMTS2 ELISA Standard Curve

Product Info

• Predicted Species Reactivity: Bos taurus, Bovine
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology ADAMTS2 ELISA Kit (Bovine) (OKEH08477) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ADAMTS2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ADAMTS2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ADAMTS2 captured in well.
• ELISA Kit Range: 78-5000pg/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 88% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=9.2% (n = 20)
  Mean Inter-assay CV%: <=12.2% (n = 20)
• ELISA Kit Component:
  • ADAMTS2 Microplate: 96 Wells (12 x 8 Well strips)
  • ADAMTS2 Lyophilized Standard: 2
  • 100X Biotinylated ADAMTS2 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Cleaves the propeptides of type I and II collagen prior to fibril assembly. Does not act on type III collagen. May also play a role in development that is independent of its role in collagen biosynthesis.
  Subcellular Location: Secreted Extracellular space Extracellular matrix
  Unigene: Bt.96865
  String: 9913.ENSBTAP00000019526
  KEGG: bta:282401
  Pfam: PF05986
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 37pg/mL
• Specificity: Natural and recombinant Bovine A disintegrin and metalloproteinase with thrombospondin motifs 2
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: ADAMTS2
• Gene Full Name: ADAM metallopeptidase with thrombospondin type 1 motif 2
• Alias Symbols: A disintegrin and metalloproteinase with thrombospondin motifs 2;a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 2;ADAM-TS 2;ADAMTS-2;ADAM-TS2;PC I-NP;pNPI;procollagen I N-proteinase;procollagen I/II amino propeptide-processing enzyme;procollagen N-endopeptidase.
• NCBI Gene Id: 282401
• Protein Name: P79331
• Description of Target: A disintegrin and metalloproteinase with thrombospondin motifs 2
• Uniprot ID: https://www.uniprot.org/uniprot/P79331
• Protein Accession #: NP_777056.1
• Nucleotide Accession #: NM_174631.2