Aviva Systems Biology will be closed in observance of Good Friday 3/29/2024. If you are in need of any assistance please email info@avivasysbio.com

Catalog No: OCOA03823
Size:20UG
Price: $179.00
SKU
OCOA03823
Availability: Domestic: within 1 week delivery | International: 1 week
Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.

ADAMTS18 Protein Lysate (OCOA03823)

Datasheets/ManualsPrintable datasheet for ADAMTS18 Protein Lysate (OCOA03823)
Product Info
Predicted Species ReactivityHomo sapiens
Product FormatLyophilized
HostHEK293T
Additional InformationComponents: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
::Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution and StorageThe lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
TagC-MYC/DDK
DescriptionADAMTS18 HEK293T cell transient overexpression lysate (as WB positive control)
Gene SymbolADAMTS18
Gene Full NameADAM metallopeptidase with thrombospondin type 1 motif 18
Alias SymbolsA disintegrin and metalloproteinase with thrombospondin motifs 18;a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 18;a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 21;ADAMTS21;disintegrin and metalloprotease-like protein;KNO2;MMCAT.
NCBI Gene Id170692
Protein NameNM_199355, NP_955387, Q8TE60
Description of TargetA disintegrin and metalloproteinase with thrombospondin motifs 18, A disintegrin and metalloproteinase with thrombospondin motifs 18 isoform 1 preproprotein [Homo sapiens], Homo sapiens ADAM metallopeptidase with thrombospondin type 1 motif 18 (ADAMTS18), transcript variant 1, mRNA
Uniprot IDhttps://www.uniprot.org/uniprot/Q8TE60
Protein Accession #https://www.ncbi.nlm.nih.gov/protein/NP_955387
Nucleotide Accession #https://www.ncbi.nlm.nih.gov/nuccore/NM_199355
Molecular Weight135 kDa
Write Your Own Review
You're reviewing:ADAMTS18 Protein Lysate (OCOA03823)
Your Rating