ADAMTS1 ELISA Kit (Rat) (OKCD02297)

Catalog No: OKCD02297

Size:96WELLS

Rat ADAMTS1 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Rat|Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	96-104%	83-92%	80-94%	82-104%
  EDTA Plasma (n=5)	92-104%	84-95%	86-93%	94-102%
  Heparin Plasma (n=5)	91-105%	83-93%	94-101%	83-95%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to A Disintegrin And Metalloproteinase With Thrombospondin 1 (ADAMTS1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to A Disintegrin And Metalloproteinase With Thrombospondin 1 (ADAMTS1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain A Disintegrin And Metalloproteinase With Thrombospondin 1 (ADAMTS1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of A Disintegrin And Metalloproteinase With Thrombospondin 1 (ADAMTS1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 78.12-5000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	98-105	102
  EDTA Plasma (n=5)	81-104	87
  Heparin Plasma(n=5)	91-98	94

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level A Disintegrin And Metalloproteinase With Thrombospondin 1 (ADAMTS1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level A Disintegrin And Metalloproteinase With Thrombospondin 1 (ADAMTS1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-ADAMTS1 Microplate: 96 Wells (12 x 8 Well strips)
  • ADAMTS1 Lyophilized Standard: 2
  • 100X Biotinylated ADAMTS1 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: 30.5 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of A Disintegrin And Metalloproteinase With Thrombospondin 1 (ADAMTS1). No significant cross-reactivity or interference between A Disintegrin And Metalloproteinase With Thrombospondin 1 (ADAMTS1) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: ADAMTS1
• Gene Full Name: ADAM metallopeptidase with thrombospondin type 1 motif, 1
• Alias Symbols: ADAMTS-1, ADAM-TS1, ADAM-TS 1, A disintegrin and metalloproteinase with thrombospondin motifs 1
• Protein Name: A disintegrin and metalloproteinase with thrombospondin motifs 1
• Description of Target: Cleaves aggrecan, a cartilage proteoglycan, at the '1683-Glu-|-Leu-1684' site (within the chondroitin sulfate attachment domain), and may be involved in its turnover. Has angiogenic inhibitor activity (By similarity). Active metalloprotease, which may be associated with various inflammatory processes as well as development of cancer cachexia. May play a critical role in follicular rupture (By similarity).
• Uniprot ID: Q9WUQ1
• Protein Accession #: NP_077376.2
• Nucleotide Accession #: NM_024400.2
• Protein Size (# AA): 967