ADAM22 ELISA Kit (Human) (OKCD00593)

Catalog No: OKCD00593 (Formerly GWB-KBBAU2)

Size:96WELLS

Human ADAM22 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	89-96%	98-105%	79-93%	78-103%
  EDTA Plasma (n=5)	99-105%	89-101%	85-93%	81-99%
  Heparin Plasma (n=5)	78-99%	94-102%	89-98%	90-98%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to A Disintegrin And Metalloprotease 22 (ADAM22). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to A Disintegrin And Metalloprotease 22 (ADAM22). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain A Disintegrin And Metalloprotease 22 (ADAM22), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of A Disintegrin And Metalloprotease 22 (ADAM22) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	79-95	88
  EDTA plasma(n=5)	90-98	94
  heparin plasma(n=5)	84-99	91

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level A Disintegrin And Metalloprotease 22 (ADAM22) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level A Disintegrin And Metalloprotease 22 (ADAM22) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-ADAM22 Microplate: 96 Wells (12 x 8 Well Strips)
  • ADAM22 Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated ADAM22 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: < 0.058 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of A Disintegrin And Metalloprotease 22 (ADAM22). No significant cross-reactivity or interference between A Disintegrin And Metalloprotease 22 (ADAM22) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: ADAM22
• Gene Full Name: ADAM metallopeptidase domain 22
• Alias Symbols: a disintegrin and metalloproteinase domain 22;ADAM 22;DEE61;disintegrin and metalloproteinase domain-containing protein 22;EIEE61;MDC2;metalloproteinase-disintegrin ADAM22-3;metalloproteinase-like, disintegrin-like, and cysteine-rich protein 2.
• NCBI Gene Id: 53616
• Protein Name: Disintegrin and metalloproteinase domain-containing protein 22
• Description of Target: Probable ligand for integrin in the brain. This is a non catalytic metalloprotease-like protein (PubMed:19692335). Involved in regulation of cell adhesion and spreading and in inhibition of cell proliferation. Neuronal receptor for LGI1.
• Uniprot ID: Q9P0K1
• Protein Accession #: NP_004185
• Nucleotide Accession #: NM_004194.4
• Protein Size (# AA): 906