Catalog No: OKEH07915
Size:96T
Price: $800.00
SKU
OKEH07915
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityPorcine, Sus scrofa
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Linearity
Sample1:21:41:81:16
serum(n=5)103-114% (n = 20)105-115% (n = 20)100-110% (n = 20)104-113% (n = 20)
EDTA plasma(n=5)99-108% (n = 20)100-110% (n = 20)81-94% (n = 20)97-97% (n = 20)
heparin plasma(n=5)82-91% (n = 20)106-115% (n = 20)81-89% (n = 20)101-110% (n = 20)
ELISA Kit PrincipleAviva Systems Biology ADAM17 ELISA Kit (Pig) (OKEH07915) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ADAM17 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ADAM17 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ADAM17 captured in well.
ELISA Kit Range78-5000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 97% (n = 20)
ELISA Kit ReproducibilityMean Intra-assay CV%: <=5.6% (n = 20)
Mean Inter-assay CV%: <=11.7% (n = 20)
ELISA Kit Component
ComponentAmount
ADAM17 Microplate96 Wells (12 x 8 Well strips)
ADAM17 Lyophilized Standard2
100X Biotinylated ADAM17 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Additional InformationFunction: Cleaves the membrane-bound precursor of TNF-alpha to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface (By similarity). Responsible for the proteolytic release of several other cell-surface proteins, including p75 TNF-receptor, interleukin 1 receptor type II, p55 TNF-receptor, transforming growth factor-alpha, L-selectin, growth hormone receptor, MUC1 and the amyloid precursor protein. Acts as an activator of Notch pathway by mediating cleavage of Notch, generating the membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). Plays a role in the proteolytic processing of ACE2.
::Subcellular Location: Membrane Single-pass type I membrane protein
::Unigene: Ssc.19668
SMR: O77636
String: 9823.ENSSSCP00000009208
Reconstitution and StorageStore as indicated in product manual.
Sensitivity23pg/mL
SpecificityNatural and recombinant Pig Disintegrin and metalloproteinase domain-containing protein 17
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolADAM17
Alias SymbolsTNF-alpha convertase;TNF-alpha-converting enzyme.
Protein NameO77636
Description of TargetDisintegrin and metalloproteinase domain-containing protein 17
Uniprot IDhttps://www.uniprot.org/uniprot/O77636
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