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Catalog No: OKCA02169
Size:96 Wells
Price: $754.00
SKU
OKCA02169
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3.5 Hours
ELISA Kit Linearity
Sample TypeDilutionMean (%)Range (%)
Serum (n=4)Neat8683-89
Serum (n=4)1:29489-99
Serum (n=4)1:410397-109
Serum (n=4)1:89995-103
ELISA Kit PrincipleAviva Systems Biology Adam17 ELISA Kit (Mouse) (OKCA02169) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ADAM17 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ADAM17 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample ADAM17 captured in well.
ELISA Kit Range15.6 pg/mL-1,000 pg/mL
ELISA Kit Recovery
Sample TypeMean (%)Range (%)
Serum (n=5)9590-100
EDTA plasma (n=4)8983-95
ELISA Kit ReproducibilityMean Intra-Assay Variation <10% CV (n=20)
Mean Inter-Assay Variation <12% CV (n=8)
ELISA Kit Component
ComponentAmount
Anti-ADAM17 Microplate96 Wells (12 x 8 Well strips)
ADAM17 Lyophilized Standard 2 x 1 ng
100X Biotinylated ADAM17 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 50 mL
Detector Antibody Diluent1 x 15 mL
Conjugate Diluent1 x 15 mL
25X Wash Buffer1 x 20 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma And Tissue Homogenates
Sensitivity3.9 pg/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolADAM17
Alias SymbolsTac, Tace, CD156b
NCBI Gene Id11491
Protein NameDisintegrin and metalloproteinase domain-containing protein 17
Description of TargetCleaves the membrane-bound precursor of TNF-alpha to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Plays a role in the proteolytic processing of ACE2.
Uniprot IDQ9Z0F8
Protein Accession #NP_001264195
Nucleotide Accession #NM_001277266
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