ADAM12 ELISA Kit (Human) (OKCD09253)

Catalog No: OKCD09253

Size:96WELLS

ADAM12 ELISA Kit (Human) (OKCD09253) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to A Disintegrin And Metalloprotease 12 (ADAM12). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to A Disintegrin And Metalloprotease 12 (ADAM12). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain A Disintegrin And Metalloprotease 12 (ADAM12), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of A Disintegrin And Metalloprotease 12 (ADAM12) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level A Disintegrin And Metalloprotease 12 (ADAM12) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level A Disintegrin And Metalloprotease 12 (ADAM12) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • ADAM12 Microplate: 96 Wells (12 x 8 Well strips)
  • ADAM12 Lyophilized Standard: 2
  • 100X ADAM12 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: < 0.057ng/mL

Target Info

• Gene Symbol: ADAM12
• Gene Full Name: ADAM metallopeptidase domain 12
• Alias Symbols: ADAM12-OT1;CAR10;disintegrin and metalloproteinase domain-containing protein 12;MCMP;MCMPMltna;meltrin-alpha;metalloprotease-disintegrin 12 transmembrane;MLTN;MLTNA.
• NCBI Gene Id: 8038
• Protein Name: Disintegrin and metalloproteinase domain-containing protein 12
• Description of Target: Involved in skeletal muscle regeneration, specifically at the onset of cell fusion. Also involved in macrophage-derived giant cells (MGC) and osteoclast formation from mononuclear precursors (By similarity).
• Uniprot ID: O43184
• Protein Accession #: NP_001275903.1
• Nucleotide Accession #: NM_001288974.1