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Catalog No: OKBB00463
Size:96 Wells
Price: $583.00
SKU
OKBB00463
Availability: Domestic: within 1-2 week delivery | International: within 1-2 week delivery
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ApplicationELISA
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration2.5 Hours
ELISA Kit PrincipleAviva's human ADAM12 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for ADAM12 has been precoated onto 96-well plates. Standards(CHO, R29-S513) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ADAM12 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human ADAM12 amount of sample captured in plate. This kit recognizes pro-ADAM12, mature ADAM12, and ADAM12/TIMP-3 complex.
ELISA Kit Range156 pg/ml - 10,000 pg/ml
ELISA Kit Reproducibility
Intra-Assay PrecisionInter-Assay Precision
Sample123123
n 161616242424
Mean (pg/mL)1.54.27.41.64.17.2
SD0.070.180.290.090.210.36
CV%4.33.95.65.15.15
ELISA Kit Component
ComponentAmount
96-Well plate Pre-coated with Anti-Human ADAM12 Antibody1 x (12 x 8 Well Strip)
Lyophilized Recombinant Human ADAM12 Standard2 x 10 ng/tube
100X Biotinylated Anti-Human ADAM12 Antibody1 x 130 uL
100X Avidin-Biotin-Peroxidase Complex (ABC)1 x 130 uL
Sample Diluent Buffer1 x 30 mL
Antibody Diluent Buffer1 x 12 mL
ABC Diluent Buffer1 x 12 mL
TMB Color Developing Agent1 x 10 mL
TMB Stop Solution1 x 10 mL
10X Wash Buffer1 x 30 mL
Reconstitution and StorageStore at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Sample TypeCell culture supernatants, serum, plasma (heparin) and urine
Sensitivity<10 pg/ml
SpecificityNatural and recombinant human ADAM12
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
ImmunogenExpression system for standard: CHO; Immunogen sequence: R29-S513
DescriptionFor quantitative detection of human ADAM12 in cell culture supernatants, serum, plasma(heparin) and urine.
Gene SymbolADAM12
Gene Full NameADAM metallopeptidase domain 12
Alias SymbolsADA12_HUMAN, ADAM 12, ADAM metallopeptidase domain 12, ADAM12, Disintegrin and metalloproteinase domain-containing protein 12, MCMP, MCMPMltna, Meltrin-alpha, MLTN, MLTNA
NCBI Gene Id8038
Protein NameDisintegrin and metalloproteinase domain-containing protein 12
Description of TargetADAM12 (Disintegrin and metalloproteinase domain-containing protein 12), also known as MLTN, is an enzyme that in humans is encoded by the ADAM12 gene. This gene encodes a member of the ADAM (a disintegrin and metalloprotease) protein family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. This gene has two alternatively spliced transcripts: a shorter secreted form and a longer membrane-bound form. The shorter form is found to stimulate myogenesis. By RT-PCR and immunoblot analyses that expression of mouse Adam12 increases during muscle regeneration, while the levels of other ADAMs remain constant. Immunofluorescence analysis revealed staining of small, newly formed muscle fibers in regenerating but not normal adult muscle cells. By using of fluorescence in situ hybridization, the ADAM12 gene is mapped to human chromosome 10q26.3.
Uniprot IDO43184
Protein Accession #NP_001275903.1
Nucleotide Accession #NM_001288974.1
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