ADAM10 ELISA Kit (Pig) (OKEH07468)

Catalog No: OKEH07468

Size:96T

Product Info

• Predicted Species Reactivity: Porcine, Sus scrofa
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  serum(n=5)	94-104% (n = 20)	105-115% (n = 20)	107-117% (n = 20)	103-112% (n = 20)
  EDTA plasma(n=5)	106-116% (n = 20)	82-91% (n = 20)	106-115% (n = 20)	100-109% (n = 20)
  heparin plasma(n=5)	103-113% (n = 20)	89-99% (n = 20)	106-116% (n = 20)	97-106% (n = 20)

• ELISA Kit Principle: Aviva Systems Biology ADAM10 ELISA Kit (Pig) (OKEH07468) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ADAM10 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ADAM10 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ADAM10 captured in well.
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 84% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=4.7% (n = 20)
  Mean Inter-assay CV%: <=5.9% (n = 20)
• ELISA Kit Component:
  • ADAM10 Microplate: 96 Wells (12 x 8 Well strips)
  • ADAM10 Lyophilized Standard: 2
  • 100X Biotinylated ADAM10 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Cleaves the membrane-bound precursor of TNF-alpha to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including heparin-binding epidermal growth-like factor, ephrin-A2, CD44, CDH2 and for constitutive and regulated alpha-secretase cleavage of amyloid precursor protein (APP). Contributes to the normal cleavage of the cellular prion protein. Involved in the cleavage of the adhesion molecule L1 at the cell surface and in released membrane vesicles, suggesting a vesicle-based protease activity. Controls also the proteolytic processing of Notch and mediates lateral inhibition during neurogenesis. Responsible for the FasL ectodomain shedding and for the generation of the remnant ADAM10-processed FasL (FasL APL) transmembrane form. Also cleaves the ectodomain of the integral membrane proteins CORIN and ITM2B. May regulate the EFNA5-EPHA3 signaling.
  Subcellular Location: Cell membrane Single-pass type I membrane protein Golgi apparatus membrane Single-pass type I membrane protein Is localized in the plasma membrane but is predominantly expressed in the Golgi apparatus and in released membrane vesicles derived likely from the Golgi.
  Unigene: Ssc.1114
  String: 9823.ENSSSCP00000004952
• Reconstitution and Storage: Store as indicated in product manual.
• Specificity: Natural and recombinant Pig Disintegrin and metalloproteinase domain-containing protein 10
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: ADAM10
• Gene Full Name: ADAM metallopeptidase domain 10
• Alias Symbols: a disintegrin and metalloproteinase domain-containing protein 10;ADAM 10;ADAM-10;disintegrin and metalloproteinase domain-containing protein 10;disintegrin-metalloproteinase;kuzbanian protein homolog;mammalian disintegrin-metalloprotease.
• NCBI Gene Id: 397345
• Protein Name: O77633
• Description of Target: Disintegrin and metalloproteinase domain-containing protein 10
• Uniprot ID: https://www.uniprot.org/uniprot/O77633
• Protein Accession #: NP_001124003.1
• Nucleotide Accession #: NM_001130531.1