ADA2 Chemi-Luminescent ELISA Kit (Human) (OKCD03366)

Catalog No: OKCD03366

Size:96WELLS

Human ADA2 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	82-90%	96-103%	78-104%	88-95%
  EDTA Plasma (n=5)	78-88%	99-105%	85-99%	88-102%
  Heparin Plasma (n=5)	78-99%	88-99%	88-102%	93-102%

• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Cat Eye Syndrome Chromosome Region, Candidate 1 (CECR1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Cat Eye Syndrome Chromosome Region, Candidate 1 (CECR1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Cat Eye Syndrome Chromosome Region, Candidate 1 (CECR1) level in the sample or standard.
• ELISA Kit Range: 27.4-20,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	78-93	83
  EDTA Plasma (n=5)	98-105	102
  Heparin Plasma (n=5)	96-103	101

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cat Eye Syndrome Chromosome Region, Candidate 1 (CECR1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cat Eye Syndrome Chromosome Region, Candidate 1 (CECR1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • ADA2 Microplate: 96 Wells (12 x 8 Well strips)
  • ADA2 Lyophilized Standard: 2
  • 100X ADA2 Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 0.313 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: ADA2
• Gene Full Name: adenosine deaminase 2
• Alias Symbols: adenosine deaminase 2;adenosine deaminase CECR1;ADGF;cat eye syndrome chromosome region, candidate 1;cat eye syndrome critical region protein 1;CECR1;IDGFL;PAN;SNEDS;VAIHS.
• NCBI Gene Id: 51816
• Protein Name: Adenosine deaminase 2
• Description of Target: Adenosine deaminase that may contribute to the degradation of extracellular adenosine, a signaling molecule that controls a variety of cellular responses. Requires elevated adenosine levels for optimal enzyme activity. Binds to cell surfaces via proteoglycans and may play a role in the regulation of cell proliferation and differentiation, independently of its enzyme activity.
• Uniprot ID: Q9NZK5
• Protein Accession #: NP_001269154
• Nucleotide Accession #: NM_001282225