Catalog No: OKCD02339
Size:96WELLS
Price: $675.00
SKU
OKCD02339
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Datasheets/ManualsPrintable datasheet for ACY1 ELISA Kit (Human) (OKCD02339)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)94-101%96-104%98-105%83-104%
EDTA Plasma (n=5)82-92%96-105%89-96%99-105%
Heparin Plasma (n=5)94-103%99-105%97-104%92-101%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Aminoacylase 1 (ACY1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Aminoacylase 1 (ACY1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Aminoacylase 1 (ACY1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Aminoacylase 1 (ACY1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)95-10399
EDTA Plasma (n=5)87-9793
Heparin Plasma(n=5)83-9086
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Aminoacylase 1 (ACY1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Aminoacylase 1 (ACY1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-ACY1 Microplate96 Wells (12 x 8 Well strips)
ACY1 Lyophilized Standard2
100X Biotinylated ACY1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Sensitivity0.055 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Aminoacylase 1 (ACY1). No significant cross-reactivity or interference between Aminoacylase 1 (ACY1) and analogues was observed.V
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolACY1
Gene Full Nameaminoacylase 1
Alias SymbolsACY-1;ACY1D;acylase;aminoacylase-1;epididymis secretory protein Li 5;HEL-S-5;N-acyl-L-amino-acid amidohydrolase.
NCBI Gene Id95
Protein NameAminoacylase-1
Description of TargetInvolved in the hydrolysis of N-acylated or N-acetylated amino acids (except L-aspartate).
Uniprot IDQ03154
Protein Accession #NP_000657.1
Nucleotide Accession #NM_000666.2
Protein Size (# AA)408
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