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Catalog No: OCOA15414
Size:20UG
Price: $275.00
SKU
OCOA15414
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ACVRL1 Protein Lysate (OCOA15414)

Datasheets/ManualsPrintable datasheet for ACVRL1 Protein Lysate (OCOA15414)
Product Info
Predicted Species ReactivityHomo sapiens
Product FormatLyophilized
HostHEK293T
Additional InformationComponents: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
::Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution and StorageThe lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
TagC-MYC/DDK
DescriptionACVRL1 HEK293T cell transient overexpression lysate (as WB positive control)
Gene SymbolACVRL1
Gene Full Nameactivin A receptor like type 1
Alias Symbolsactivin A receptor type II-like 1;activin A receptor type IL;activin A receptor, type II-like kinase 1;Activin receptor-like kinase 1;ACVRLK1;ALK1;ALK-1;HHT;HHT2;ORW2;serine/threonine-protein kinase receptor R3;SKR3;TGF-B superfamily receptor type I;TSR-I.
NCBI Gene Id94
Protein NameA0A0S2Z310, NM_001077401, NP_001070869, P37023
Description of TargetHomo sapiens activin A receptor like type 1 (ACVRL1), transcript variant 2, mRNA, Serine/threonine-protein kinase receptor, Serine/threonine-protein kinase receptor R3, serine/threonine-protein kinase receptor R3 precursor [Homo sapiens]
Uniprot IDhttps://www.uniprot.org/uniprot/P37023
Protein Accession #https://www.ncbi.nlm.nih.gov/protein/NP_001070869
Nucleotide Accession #https://www.ncbi.nlm.nih.gov/nuccore/NM_001077401
Molecular Weight55.9 kDa
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