Catalog No: OKEH01975
Size:96T
Price: $625.00
SKU
OKEH01975
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology ACVR2A ELISA Kit (Mouse) (OKEH01975) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ACVR2A has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ACVR2A is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ACVR2A captured in well.
ELISA Kit Range0.312-20ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 102%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.3% (n = 20)
Mean Inter-assay CV%: < 8.2% (n = 20)
ELISA Kit Component
ComponentAmount
ACVR2A Microplate96 Wells (12 x 8 Well strips)
ACVR2A Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated ACVR2A Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity0.156 ng/mL
SpecificityNatural and recombinant Mouse Activin receptor type-2A
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolAcvr2a
Gene Full Nameactivin receptor IIA
Alias SymbolsAc;activin receptor type IIA;activin receptor type-2A;ActrIIa;ACTR-IIA;Acvr2;tAc;TactrII.
NCBI Gene Id11480
Protein NameP27038
Description of TargetActivin receptor type-2A
Uniprot IDhttps://www.uniprot.org/uniprot/P27038
Protein Accession #NP_031422.3
Nucleotide Accession #NM_007396.4
Protein Size (# AA)513
Molecular Weight57 kDa
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