Size:96 Wells
$599.00
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SKU
OKCD02338
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ACVR2A ELISA Kit (Human) (OKCD02338)

Catalog#: OKCD02338
Domestic: within 1-2 weeks delivery | International: 1-2 weeks
More Information
Predicted Species Reactivity Human
Kit Detection Method Colorimetric, OD450 nm
Kit Duration 3 Hours
Kit Principle Aviva Systems Biology ACVR2A ELISA Kit (Human) (OKCD02338) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ACVR2A has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wellss, incubated and removed.A biotinylated detector antibody specific for ACVR2A is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample ACVR2A captured in well.
Kit Range 0.156-10 ng/mL
Kit Reproducibility Intra-assay Precision: 3 samples with known low, middle and high level ACVR2A was tested with 20 replicates across one plate.
Inter-assay Precision: 3 samples with known low, middle and high level ACVR2A was tested on three different plates with 8 replicates, respectively.
Intra-Assay CV: <10% (n=20)
Inter-Assay CV: <12% (n=24)
Kit Component
ComponentAmount
Anti-ACVR2A Microplate96 Wells (12 x 8 Well strips)
ACVR2A Lyophilized Standard2
100X Biotinylated ACVR2A Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and Storage Store as indicated in product manual.
Sample Type Tissue homogenates, cell lysates and other biological fluids
Sensitivity 0.055 ng/mL
Datasheets/Manuals Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Specificity This assay has high sensitivity and excellent specificity for detection of Activin A Receptor Type II A (ACVR2A). No significant cross-reactivity or interference between Activin A Receptor Type II A (ACVR2A) and analogues was observed.
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Gene Symbol ACVR2A
Official Gene Full Name activin A receptor type 2A
Alias Symbols Activin receptor type-2A, Activin receptor type IIA, ACTRII, ACTRIIA, ACTR-IIA, ACVR2
NCBI Gene Id 92
Protein Name Activin receptor type-2A
Description of Target On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Receptor for activin A, activin B and inhibin A. Mediates induction of adipogenesis by GDF6.
Swissprot Id P27037
Protein Accession # NP_001265508.1
Nucleotide Accession # NM_001278579.1
Protein Size (# AA) 513
Tissue Tool Find tissues and cell lines supported by DNA array analysis to express ACVR2A.
RNA Seq Find tissues and cell lines supported by RNA-seq analysis to express ACVR2A.
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