ACVR2A ELISA Kit (Human) (OKCD02338)

Catalog No: OKCD02338

Size:96WELLS

Human ACVR2A ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Activin A Receptor Type II A (ACVR2A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Activin A Receptor Type II A (ACVR2A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Activin A Receptor Type II A (ACVR2A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Activin A Receptor Type II A (ACVR2A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Activin A Receptor Type II A (ACVR2A) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Activin A Receptor Type II A (ACVR2A) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-ACVR2A Microplate: 96 Wells (12 x 8 Well strips)
  • ACVR2A Lyophilized Standard: 2
  • 100X Biotinylated ACVR2A Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: 0.055 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Activin A Receptor Type II A (ACVR2A). No significant cross-reactivity or interference between Activin A Receptor Type II A (ACVR2A) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: ACVR2A
• Gene Full Name: activin A receptor type 2A
• Alias Symbols: activin A receptor, type IIA;Activin receptor type IIA;activin receptor type-2A;ACTRII;ACVR2.
• NCBI Gene Id: 92
• Protein Name: Activin receptor type-2A
• Description of Target: On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Receptor for activin A, activin B and inhibin A. Mediates induction of adipogenesis by GDF6 (By similarity).
• Uniprot ID: P27037
• Protein Accession #: NP_001265508.1
• Nucleotide Accession #: NM_001278579.1
• Protein Size (# AA): 513