Catalog No: OKCD00262 (Formerly GWB-KBBHH2)
Size:96 Wells
Price: $650.00
SKU
OKCD00262
Availability: Domestic: within 2-3 weeks delivery | International: 2-3 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ApplicationELISA
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration2.5 Hours
ELISA Kit PrincipleAviva Systems Biology ACVR1 ELISA Kit (Human) (OKCD00262) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ACVR1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ACVR1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample ACVR1captured in well.
ELISA Kit Range0.156-10 ng/mL
ELISA Kit ReproducibilityIntra-assay Precision: 3 samples with known low, middle and high level ACVR1 were tested with 20 replicates across one plate.
Inter-assay Precision: 3 samples with known low, middle and high level ACVR1 were tested on three different plates with 8 replicates, respectively.
Intra-Assay CV: <10%
Inter-Assay CV: <12%
ELISA Kit Component
ComponentAmount
Anti-ACVR1 Microplate96 Wells (12 x 8 Well Strips)
ACVR1 Lyophilized Standard2 x 10 ng
100X Biotinylated ACVR1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeTissue homogenates and other biological fluids.
Sensitivity< 0.063 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Activin A Receptor Type I (ACVR1). No significant cross-reactivity or interference between Activin A Receptor Type I (ACVR1) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolACVR1
Gene Full Nameactivin A receptor type 1
Alias SymbolsFOP, ALK2, SKR1, TSRI, ACTRI, ACVR1A, ACVRLK2
NCBI Gene Id90
Protein Nameactivin receptor type-1
Description of TargetOn ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Receptor for activin. May be involved for left-right pattern formation during embryogenesis (By similarity).By similarity
Swissprot IdQ04771
Protein Accession #NP_001096
Nucleotide Accession #NM_001105.4
Protein Size (# AA)509
Tissue ToolFind tissues and cell lines supported by DNA array analysis to express ACVR1.
RNA SeqFind tissues and cell lines supported by RNA-seq analysis to express ACVR1.
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