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Catalog No: OKEH01032
Price: $525.00
Availability: Domestic: within 2-3 weeks delivery | International: 2-3 weeks
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ACTN4 ELISA Kit (Human) : 96 Wells (OKEH01032)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology ACTN4 ELISA Kit (Human) (OKEH01032) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ACTN4 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ACTN4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ACTN4 captured in well.
ELISA Kit Range78-5000pg/ml
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 98%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.7% (n = 20)
Mean Inter-assay CV%: < 8.1% (n = 20)
ELISA Kit Component
ACTN4 Microplate96 Wells (12 x 8 Well strips)
ACTN4 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated ACTN4 Detector Antibody1 x 120 ul
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity32 pg/mL
SpecificityNatural and recombinant Human Alpha-actinin-4
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolACTN4
Gene Full Nameactinin alpha 4
Alias SymbolsACTININ-4;alpha-actinin-4;focal segmental glomerulosclerosis 1;FSGS;FSGS1;non-muscle alpha-actinin 4.
NCBI Gene Id81
Protein NameO43707
Description of TargetAlpha-actinin-4
Uniprot ID
Protein Accession #NP_004915.2
Nucleotide Accession #NM_004924.4
Protein Size (# AA)911
Molecular Weight104 kDa
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