ACTN4 Antibody - N-terminal region (ARP42202_T100)

Catalog No: ARP42202_T100

WB Suggested Anti-ACTN4 Antibody Titration: 0.625ug/ml
Positive Control: HCT116 cell lysateACTN4 is supported by BioGPS gene expression data to be expressed in HCT116

Lanes:
Lane1: 10 ug ACTN1-GFP transfected COS-7 lysate
Lane2: 10 ug ACTN2-GFP transfected COS-7 lysate
Lane3: 10 ug ACTN3-GFP transfected COS-7 lysate
Lane4: 10 ug ACTN4-GFP transfected COS-7 lysate
Primary Antibody Dilution:
1: 1000
Secondary Antibody:
Anti-rabbit HRP
Secondary Antibody Dilution:
1:5000
Gene Name:
ACTN4
Submitted by:
Johannes W. Hell, University of California

Sample Type:
ACTNX-GFP transfected COS-7 cells
Primary Antibody Dilution:
1:1000
Secondary Antibody:
Anti-rabbit-Alexa-Fluor 568
Secondary Antibody Dilution:
1:100
Color/Signal Descriptions:
Green: GFP Red: ACTN4
Gene Name:
ACTN4
Submitted by:
Johannes W. Hell, Ph.D., Professor, Department of Pharmacology, University of California, Room 2419D Tupper Hall, Davis, CA 95616-5270, Phone: (530) 752 6540, Lab: (530) 752 7551, FAX: (530) 752 7710

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Clonality: Polyclonal
• Host: Rabbit
• Application: IHC, WB
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human ACTN4
• Purification: Protein A purified
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 93%
• Peptide Sequence: Synthetic peptide located within the following region: LIHRHRPELIEYDKLRKDDPVTNLNNAFEVAEKYLDIPKMLDAEDIVNTA
• Concentration: 1.0 mg/ml
• Blocking Peptide: For anti-ACTN4 (ARP42202_T100) antibody is Catalog # AAP42202 (Previous Catalog # AAPP24625)
• Sample Type Confirmation:

  ACTN4 is supported by BioGPS gene expression data to be expressed in HCT116

• Reference: Kimura,K., (2006) Genome Res. 16 (1), 55-65
• Publications:

  Wei, S., Gao, X., Du, J., Su, J. & Xu, Z. Angiogenin enhances cell migration by regulating stress fiber assembly and focal adhesion dynamics. PLoS One 6, e28797 (2011). 22194915

Target Info

• Gene Symbol: ACTN4
• Gene Full Name: Actinin, alpha 4
• Alias Symbols: FSGS, FSGS1, ACTININ-4
• NCBI Gene Id: 81
• Protein Name: Alpha-actinin-4
• Description of Target: Alpha actinins belong to the spectrin superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. ACTN4 is a nonmuscle, alpha actinin isoform which is concentrated in the cytoplasm, and thought to be involved in metastatic processes. Mutations in its gene have been associated with focal and segmental glomerulosclerosis.Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, alpha actinin isoform which is concentrated in the cytoplasm, and thought to be involved in metastatic processes. Mutations in this gene have been associated with focal and segmental glomerulosclerosis.
• Uniprot ID: O43707
• Protein Accession #: NP_004915
• Nucleotide Accession #: NM_004924
• Protein Size (# AA): 911
• Molecular Weight: 100kDa
• Protein Interactions: MYOZ2; HUWE1; UBC; BMP7; ACTN3; SUMO2; SUMO3; GNB2; SUMO1; NEDD8; MDM2; HGS; SF3A3; VCP; CDKN2A; ACTN1; PDLIM1; UBD; IGSF8; ICAM1; CD81; SVIL; Fcho2; POT1; VCAM1; TXN; RPS27; MAPK7; KPNA2; ITGA4; FN1; ESR1; CDH1; TRAF3IP1; FMNL1; NOS3; ACTN2; UBASH3B; SHC