ACTN1 Antibody (OAAD00214)

Catalog No: OAAD00214

Size:100 ug

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Clone: SA-20
• Isotype: IgG1
• Host: Mouse
• Application: WB, IHC, IP
• Reconstitution and Storage: At -20C for one year. After reconstitution, at 4C for one month. It can also be aliquotted and stored frozen at -20C for longer time.
• Immunogen: Rabbit skeletal alpha-actinin
• Purification: Purified by the goat anti-mouse IgG Affinity chromatography.
• Specificity: Human, mouse, rat.
  No cross reactivity with other proteins.
• Formulation: Lyophilized from 1.2% sodium acetate, with 2mg BSA and 0.01mg NaN3 as preservative.
• Application Info: Western blot At 3.5ug/ml with the appropriate system to detect alpha-actinin in cells and tissues.
  Immunohistochemistry(P) At 3.5-7ug/ml to detect alpha-actinin in formalin fixed and paraffin embedded tissues. Boiling the sections is required.
  Immunohistochemistry(F) At 3.5-7ug/ml to detect alpha-actinin in formalin or acetone fixed tissues.
  Other applications have not been tested.
  Optimal dilutions should be determined by end user.
• Storage Buffer: 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100ug/ml.
• Reference: Youssoufian, H.; McAfee, M.; Kwiatkowski, D. J. : Cloning and chromosomal localization of the human cytoskeletal alpha-actinin gene reveals linkage to the beta-spectrin gene. Am. J. Hum. Genet. 47: 62-72, 1990.

Target Info

• Gene Symbol: ACTN1
• Gene Full Name: Alpha-actinin-1
• Alias Symbols: BDPLT15
• NCBI Gene Id: 87
• Protein Name: Alpha-actinin-1
• Description of Target: Alpha-actinin was initially isolated from rabbit skeletal muscle as a factor that induces the gelation of F-actin and promotes the superprecipitation of actomyosin. Alpha actinins are actin-binding proteins that carry out different purposes in different different cell types. In myofibrillar cells, alpha-actinin constitutes a major component of Z-discs in striated muscle and of the functionally analogous dense bodies and dense plaques in smooth muscle. alpha-actinin (alpha A) shares structural homology with spectrin and dystrophin.
• Uniprot ID: P12814
• Protein Accession #: NP_001093.1; NP_001123476.1; NP_001123477.1
• Nucleotide Accession #: NM_001102.3; NM_001130004.1; NM_001130005.1