Catalog No: OCOA13179
Size:20UG
Price: $179.00
SKU
OCOA13179
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ACTL6A Protein Lysate (OCOA13179)

Datasheets/ManualsPrintable datasheet for ACTL6A Protein Lysate (OCOA13179)
Product Info
Predicted Species ReactivityHomo sapiens
Product FormatLyophilized
HostHEK293T
Additional InformationComponents: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
::Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution and StorageThe lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
TagC-MYC/DDK
DescriptionACTL6A HEK293T cell transient overexpression lysate (as WB positive control)
Gene SymbolACTL6A
Gene Full Nameactin like 6A
Alias Symbols53 kDa BRG1-associated factor A;actin-like protein 6A;actin-related protein 4;actin-related protein Baf53a;ACTL6;Arp4;arpNbeta;ARPN-BETA;BAF complex 53 kDa subunit;BAF53;BAF53A;BRG1-associated factor 53A;hArpN beta;INO80 complex subunit K;INO80K;SMARCN1.
NCBI Gene Id86
Protein NameNM_004301, NP_004292, O96019
Description of TargetActin-like protein 6A, actin-like protein 6A isoform 1 [Homo sapiens], Homo sapiens actin like 6A (ACTL6A), transcript variant 1, mRNA
Uniprot IDhttps://www.uniprot.org/uniprot/O96019
Protein Accession #https://www.ncbi.nlm.nih.gov/protein/NP_004292
Nucleotide Accession #https://www.ncbi.nlm.nih.gov/nuccore/NM_004301
Molecular Weight43.25 kDa
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