Activin A ELISA Kit (Rat) (OKBB00125)

Catalog No: OKBB00125

Size:96 Tests

Rat INHBA ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva's rat Activin A ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Activin A has been precoated onto 96-well plates. Standards(CHO, G311-S426) and test samples are added to the wells, a biotinylated detection monoclonal antibody from mouse specific for Activin A is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the rat Activin A amount of sample captured in plate.
• ELISA Kit Range: 15.6 pg/ml - 1,000 pg/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  Mean (pg/mL)	97	294	459	103	315	512
  St. Dev.	4.9	14.7	23.9	8	19.8	24.6
  %CV	5.05	5	5.20	7.76	6.28	4.80

• ELISA Kit Component:
  • Lyophilized recombinant rat Activin A standard: 10 ng/tube x 2
  • Anti-rat Activin A Antibody Well Plate: 96 Wells
  • Sample diluent buffer: 30 mL
  • Biotinylated anti-rat Activin A antibody: 130 uL, dilution 1:100
  • Antibody diluent buffer: 12 mL
  • Avidin-Biotin-Peroxidase Complex (ABC): 130 uL, dilution 1:100
  • ABC diluent buffer: 12 mL
  • TMB color developing agent: 10 mL
  • TMB stop solution: 10 mL
• Additional Information: Range: 62.5pg/ml-4000pg/ml
  Principle: Aviva’s rat Activin A ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. Rat Activin A specific-specific monoclonal antibodies were precoated onto 96-well plates. The rat specific detection monoclonal antibodies were biotinylated. The test samples and biotinylated detection antibodies were added to the wells subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat Activin A amount of sample captured in plate.
  Notes: 1. To inspect the validity of experiment operation and the appropriateness of sample dilution proportion, pilot
  experiment using standards and a small number of samples is recommended.
  2. The TMB Color Developing agent is colorless and transparent before using, contact us freely if it is not the case.
  3. Before using the Kit, spin tubes and bring down all components to the bottom of tubes.
  4. Duplicate well assay is recommended for both standard and sample testing.
  5. Don’t let 96-well plate dry, for dry plate will inactivate active components on plate.
  6. Don’t reuse tips and tubes to avoid cross contamination.
  7. To avoid to use the reagents from different batches together.
  8. In order to avoid marginal effect of plate incubation due to temperature difference ( reaction may be stronger in the
  marginal wells), it is suggested that the diluted ABC and TMB solution will be pre-warmed in 37C for 30 min before
  using.
  Background: Activin A is a homodimer of 14kDa beta-A. Activin A, a cytokine member of the transforming growth factor-beta superfamily, is expressed locally by the mesenchymal component of the hemopoietic microenvironment. Its expression is regulated on the mRNA level by different cytokines, and the biological activity of the protein is tightly controlled by several inhibitory molecules.1 Inhibins and activins are members of the transforming growth factor beta superfamily and are known to modulate the growth and differentiation of several cell types.2 Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins. The standard product used in this kit is recombinant Activin A, which is composed of two single chains of 116 amino acids with the molecular mass of 26KDa.
• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: cell culture supernates, serum, plasma (heparin, EDTA) and saliva
• Sensitivity: <12 pg/ml
• Predicted Homology Based on Immunogen Sequence: No detectable cross-reactivity with any other cytokine.
• Reference: 1. Shav-Tal, Y.; Zipori, D. The role of activin a in regulation of hemopoiesis. Stem Cells. 2002;20(6):493-500.
  2. Di, Loreto, C.; Reis, FM.; Cataldi, P.; Zuiani, C.; Luisi, S.; Beltrami, CA.; Petraglia, F. Human mammary gland and
  breast carcinoma contain immunoreactive inhibin/activin subunits: evidence for a secretion into cystic fluid. Eur J
  Endocrinol.1999 Aug;141(2):190-4.
• Specificity: Natural and recombinant rat Activin A
• Immunogen: Expression system for standard: CHO; Immunogen sequence: G311-S426
• Description: For quantitative detection of rat Activin A in cell culture supernatants, serum, plasma(heparin, EDTA) and saliva.

Target Info

• Gene Symbol: INHBA
• Gene Full Name: inhibin, beta A
• Alias Symbols: Activin beta A chain, erythroid differentiation factor, erythroid differentiation protein, follicle stimulating hormone releasing protein, FSH releasing protein, Inhibin, inhibin beta A chain
• NCBI Gene Id: 29200
• Protein Name: Inhibin beta A chain
• Description of Target: Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins.
• Uniprot ID: P18331
• Protein Accession #: NP_058824.1
• Nucleotide Accession #: NM_017128.2