ACTH ELISA Kit (Human) (OKEH00625)

Catalog No: OKEH00625

Size:96 Wells

Human ACTH ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology ACTH ELISA Kit (Human) (OKEH00625) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ACTH has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ACTH is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ACTH captured in well.
• ELISA Kit Range: 0.39 - 25ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 104%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 6.5% (n = 20)
  Mean Inter-assay CV%: < 10.8% (n = 20)
• ELISA Kit Component:
  • ACTH Microplate: 96 Wells (12 x 8 Well strips)
  • ACTH Lyophilized Standard: 2
  • 100X Biotinylated ACTH Detector Antibody: 1 x 120 ÂuL
  • 100X Avidin-HRP Conjugate: 1 x 120 ÂuL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Urine, Tissue homogenates, Cell culture supernates, Other biological fluids
• Sensitivity: 0.10 ng/mL
• Specificity: Natural and recombinant Human Corticotropin
• Publications:

  Nicotinamide phosphoribosyltransferase and the hypothalamic-pituitary-adrenal axis of the rat. Mol Med Rep. 17, 6163-6173 (2018). 29436637

• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: ACTH
• Gene Full Name: proopiomelanocortin
• Alias Symbols: CRF, CRH1
• NCBI Gene Id: 1392
• Protein Name: Pro-opiomelanocortin
• Description of Target: This gene encodes a preproprotein that undergoes extensive, tissue-specific, post-translational processing via cleavage by subtilisin-like enzymes known as prohormone convertases. There are eight potential cleavage sites within the preproprotein and, depending on tissue type and the available convertases, processing may yield as many as ten biologically active peptides involved in diverse cellular functions. The encoded protein is synthesized mainly in corticotroph cells of the anterior pituitary where four cleavage sites are used; adrenocorticotrophin, essential for normal steroidogenesis and the maintenance of normal adrenal weight, and lipotropin beta are the major end products. In other tissues, including the hypothalamus, placenta, and epithelium, all cleavage sites may be used, giving rise to peptides with roles in pain and energy homeostasis, melanocyte stimulation, and immune modulation. These include several distinct melanotropins, lipotropins, and endorphins that are contained within the adrenocorticotrophin and beta-lipotropin peptides. The antimicrobial melanotropin alpha peptide exhibits antibacterial and antifungal activity. Mutations in this gene have been associated with early onset obesity, adrenal insufficiency, and red hair pigmentation. Alternatively spliced transcript variants encoding the same protein have been described.
• Uniprot ID: P01189
• Protein Accession #: NP_000930.1
• Nucleotide Accession #: NM_000939.3
• Protein Size (# AA): 196
• Molecular Weight: 21 kDa