ACTC1 Antibody (OAGA00135)

Catalog No: OAGA00135

Size:100 ul

Sample(30 ?g of whole cell lysate)
A:HeLa S3
B:MOLT4
12% SDS PAGE
OAGA00135 diluted at 1:500

Sample (30 ug of whole cell lysate)
A: NIH-3T3
10% SDS PAGE
OAGA00135 diluted at 1:1000

alpha Actin (cardiac muscle) antibody detects alpha Actin (cardiac muscle) protein by western blot analysis.
A. 30 ?g PC-12 whole cell lysate/extract
B. 30 ?g Rat2 whole cell lysate/extract
10 % SDS-PAGE
alpha Actin (cardiac muscle) antibody (OAGA00135) dilution: 1:10000

Immunohistochemical analysis of paraffin-embedded Gastric CA, using alpha Actin (cardiac muscle)(OAGA00135) antibody at 1:100 dilution.

alpha Actin (cardiac muscle) antibody detects alpha Actin (cardiac muscle) protein at cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in 0.5% Triton X-100 for 1 min, then ice-cold methanol for 5 min.
Green: alpha Actin (cardiac muscle) protein stained by alpha Actin (cardiac muscle) antibody (OAGA00135) diluted at 1:200.
Blue: Hoechst 33342 staining.

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Liquid. PBS with 1% BSA, 20% Glycerol (pH 7) and 0.01% Thimerosal as a preservative.
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: IF, ICC, IHC-FFPE, WB
  Positive Controls: 293T, A431, H1299, HeLaS3, HepG2, Molt-4, Raji, NIH-3T3, PC-12, Rat2
  Cellular Localization: Cytoplasm, cytoskeleton.
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant protein fragment contain a sequence corresponding to a region within amino acids 99 and 354 (P68032) of alpha Actin (cardiac muscle).
• Purification: Purified by antigen-affinity chromatography.
• Predicted Homology Based on Immunogen Sequence: Xenopus laevis (99%), Pig (100%), Chicken (100%), Bovine (100%)
• Concentration: 1 mg/ml
• Application Info: ICC/IF: 1:100~1000
  IHC-FFPE: 1:100~1000
  WB: 1:500~10000
  

Target Info

• Gene Symbol: ACTC1
• Gene Full Name: Actin, alpha cardiac muscle 1
• Alias Symbols: ACTC, ASD5, CMD1R, CMH11, LVNC4
• NCBI Gene Id: 70
• Protein Name: Actin, alpha cardiac muscle 1
• Description of Target: Actins are highly conserved proteins that are involved in various types of cell motility. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to four others. The protein encoded by this gene belongs to the actin family which is comprised of three main groups of actin isoforms, alpha, beta, and gamma. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. Defects in this gene have been associated with idiopathic dilated cardiomyopathy (IDC) and familial hypertrophic cardiomyopathy (FHC).
• Uniprot ID: P68032
• Protein Accession #: NP_005150.1
• Molecular Weight: 42 kDa