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- $55: Antibody & Protein in US
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Datasheets/Manuals | Printable datasheet for ACTB Antibody (OASA02777) |
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Predicted Species Reactivity | Human |
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Product Format | Liquid. Phosphate buffered saline |
Clonality | Monoclonal |
Clone | NH3 |
Isotype | IgM |
Host | Mouse |
Application | Enzyme-linked immunosorbent assay|Flow cytometry|Immunohistochemistry-Frozen|Western blot |
Additional Information | Fusion Partners: Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line. |
:: | Preparation: Purified IgM prepared from tissue culture supernatant Preservative Stabilisers: 0.09% - Sodium Azide |
:: | Approx Protein Conc: IgM concentration 1.0 mg/ml Buffer Solutions: Phosphate buffered saline pH7.4 |
Reconstitution and Storage | -20°C |
Immunogen | Human monocytes and U937 cell line. |
Purification | Purified IgM prepared from tissue culture supernatant |
Predicted Homology Based on Immunogen Sequence | Human |
Concentration | 1 mg/ml |
Specificity | ACTIN F TYPE |
Application Info | Flow Cytometry: 1 / 10 ELISA: 1 / 10 Western Blotting: 1/100 - 1/500 |
Reference | 1. Dransfield, I. et al. (1988) Initial characterization of an anti-actin monoclonal antibody (NH3). Biochem. Soc. Trans. 16: 163-164. 2. McCarthy, A.M. et al. (2006) Loss of cortical actin filaments in insulin-resistant skeletal muscle cells impairs GLUT4 vesicle trafficking and glucose transport. Am J Physiol Cell Physiol. 291: C860-8. |
Gene Symbol | ACTB |
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Alias Symbols | BRWS1, PS1TP5BP1 |
NCBI Gene Id | 60 |
Protein Name | Actin, cytoplasmic 1 |
Description of Target | MOUSE ANTI HUMAN F-ACTIN |
Uniprot ID | P60709 |
Protein Accession # | NP_001092.1 |
Protein Size (# AA) | 375 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "ACTB Antibody (OASA02777)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human".
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How long will it take to receive "ACTB Antibody (OASA02777)"?
This item is available "Domestic: within 1-2 weeks delivery | International: 1-2 weeks".
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What buffer format is "ACTB Antibody (OASA02777)" provided in?
This item is provided in "Liquid. Phosphate buffered saline".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "ACTB Antibody (OASA02777)"?
This target may also be called "BRWS1, PS1TP5BP1" in publications.
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What is the shipping cost for "ACTB Antibody (OASA02777)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "ACTB Antibody (OASA02777)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "ACTB Antibody (OASA02777)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "ACTB Antibody (OASA02777)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "ACTB"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "ACTB"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "ACTB"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "ACTB"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "ACTB"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "ACTB"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.