ACTB Antibody (OAAF00923)

Catalog No: OAAF00923 (Formerly GWB-ASC337)

Size:100 ug

Western blot analysis of extracts from mouse brain cells, using Actin-pan Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human skeletal muscle tissue, using Actin-pan Antibody. The picture on the right is treated with the synthesized peptide.

Immunofluorescence analysis of HuvEc cells, using Actin-pan Antibody. The picture on the right is treated with the synthesized peptide.

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Liquid. PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot
• Reconstitution and Storage: -20°C
• Immunogen: The antiserum was produced against synthesized peptide derived from human Actin.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Specificity: ACTB antibody detects endogenous levels of total ACTB protein.
• Dilution: The optimal working dilution should be determined by the end user.
• Application Info: WB 1:500~1000
  IHC 1:50~100
  IF 1:100~500
  ELISA 1:10000
  
• Reference: A. J. MacQueen, Mol. Biol. Cell, Jul 2005; 16: 3247 - 3259.
  Isaac Rabinovitz, J. Cell Biol., Dec 1997; 139: 1873.
  Deanne H. Hryciw, Am J Physiol Renal Physiol, Jun 2005; 288: F1227 - F1235.
  Derek T. Warren, J. Cell Sci., Apr 2002; 115: 1703.

Target Info

• Gene Symbol: ACTA1|ACTC1|ACTG1|ACTG2
• Gene Full Name: actin alpha 1, skeletal muscle|actin alpha cardiac muscle 1|actin gamma 1|actin gamma 2, smooth muscle
• Alias Symbols: ACT;ACTA;ACTA3;ACTC;ACTE;ACTG;actin, alpha cardiac muscle 1;actin, alpha skeletal muscle;actin, cytoplasmic 2;actin, gamma 2, smooth muscle, enteric;actin, gamma-enteric smooth muscle;actin-like protein;ACTL3;ACTSG;Alpha-actin-1;alpha-actin-3;Alpha-cardiac actin;ASD5;ASMA;CFTD;CFTD1;CFTDM;CMD1R;CMH11;cytoskeletal gamma-actin;DFNA20;DFNA26;epididymis luminal protein 176;Gamma-2-actin;Gamma-actin;HEL-176;LVNC4;MMIHS5;MPFD;NEM1;NEM2;NEM3;nemaline myopathy type 3;SHPM;Smooth muscle gamma-actin;VSCM;VSCM1.
• NCBI Gene Id: 58|70|71|72
• Protein Name: Actin, alpha cardiac muscle 1|Actin, alpha skeletal muscle|Actin, cytoplasmic 2|Actin, gamma-enteric smooth muscle
• Description of Target: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
• Uniprot ID: P63261|P63267|P68032|P68133
• Protein Accession #: NP_001186883.1
• Molecular Weight: 41 kDa