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ACTB Antibody (OAAD00224)

100 ug
$255.00
In Stock
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Gene Symbol:
ACTB
NCBI Gene Id:
60
Official Gene Full Name:
Actin, cytoplasmic 1
Protein Name:
Actin, cytoplasmic 1
Swissprot Id:
P60709
Protein Accession #:
NP_001092.1
Nucleotide Accession #:
NM_001101.3
Replacement Item:
This antibody may replace item sc-10731 from Santa Cruz Biotechnology.
Description of Target:
The primary site of action of cytochalasin B on cell motility processes is beta-actin. Habets et al. (1992) generated hybrids that harbor only specific regions of human chromosome 7 and assigned the ACTB locus to 7p15-p12. ACTB and the other assigned beta-actin-related sequences are dispersed over at least four different chromosomes including one locus assigned to the X chromosome. A mutation of beta-actin that alters depolymerization dynamics is associated with autosomal dominant developmental malformations, deafness, and dystonia.
Host:
Mouse
Clonality:
Monoclonal
Purification:
Purified by the goat anti-mouse IgG Affinity chromatography.
Application:
WB, IHC, IP
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express ACTB.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express ACTB.
Immunogen:
slightly modified beta-cytoplasmic actin N-terminal peptide, Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys, conjugated to KLH.
Product Format:
Liquid. In PBS Buffer.
Reconstitution and Storage:
At -20C for one year. At 4C for three month. It can also be aliquotted and stored frozen at -20C for longer time.
Datasheets/Manuals:
Printable datasheet for anti-ACTB (OAAD00224) antibody
Specificity:
Human, mouse, rat.
No cross reactivity with other proteins.
Clone:
AC-15
Isotype:
IgG1
Application Info:
Western blot At 0.25-0.5ug/ml with the appropriate system to detect beta-actin in cells and tissues.
Immunohistochemistry(P) At 0.5-1ug/ml to detect beta-actin in formalin fixed and paraffin embedded tissues.
Other applications have not been tested.
Optimal dilutions should be determined by end user.
Publications:

Martinel Lamas, DJ; Nicoud, MB; Sterle, HA; Carabajal, E; Tesan, F; Perazzo, JC; Cremaschi, GA; Rivera, ES; Medina, VA; Selective cytoprotective effect of histamine on doxorubicin-induced hepatic and cardiac toxicity in animal models. 1, 15059 (2015). WB, IHC, IP, Human, Mouse, Rat 27551485

Target Reference:
1. Ng, S.-Y.; Gunning, P.; Eddy, R.; Ponte, P.; Leavitt, J.; Kedes, L.; Shows, T. : Chromosome 7 assignment of the human beta-actin functional gene (ACTB) and the chromosomal dispersion of pseudogenes. (Abstract) Cytogenet. Cell Genet. 40: 712 only, 1985.
2. Procaccio, V.; Salazar, G.; Ono, S.; Styers, M. L.; Gearing, M.; Davila, A.; Jimenez, R.; Juncos, J.; Gutekunst, C.-A.; Meroni, G.; Fontanella, B.; Sontag, E.; Sontag, J. M.; Faundez, V.; Wainer, B. H. : A mutation of beta-actin that alters depolymerization dynamics is associated with autosomal dominant developmental malformations, deafness, and dystonia. Am. J. Hum. Genet. 78: 947-960, 2006.
3. Ng, S.-Y.; Gunning, P.; Eddy, R.; Ponte, P.; Leavitt, J.; Shows, T.; Kedes, L. : Evolution of the functional human beta-actin gene and its multi-pseudogene family: conservation of the noncoding regions and chromosomal dispersion of pseudogenes. Molec. Cell. Biol. 5: 2720-2732, 1985.

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