Size:100 ug
$255.00
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SKU
OAAD00224
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ACTB Antibody (OAAD00224)

Catalog#: OAAD00224
Domestic: within 1 week delivery | International: 1 week
More Information
Predicted Species Reactivity Human, Mouse, Rat
Product Format Liquid. In PBS Buffer.
Clonality Monoclonal
Clone AC-15
Isotype IgG1
Host Mouse
Application WB, IHC, IP
Reconstitution and Storage At -20C for one year. At 4C for three month. It can also be aliquotted and stored frozen at -20C for longer time.
Replacement Item This antibody may replace item sc-10731 from Santa Cruz Biotechnology.
Immunogen slightly modified beta-cytoplasmic actin N-terminal peptide, Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys, conjugated to KLH.
Purification Purified by the goat anti-mouse IgG Affinity chromatography.
Datasheets/Manuals Printable datasheet for anti-ACTB (OAAD00224) antibody
Specificity Human, mouse, rat.
No cross reactivity with other proteins.
Application Info Western blot At 0.25-0.5ug/ml with the appropriate system to detect beta-actin in cells and tissues.
Immunohistochemistry(P) At 0.5-1ug/ml to detect beta-actin in formalin fixed and paraffin embedded tissues.
Other applications have not been tested.
Optimal dilutions should be determined by end user.
Publications

Martinel Lamas, DJ; Nicoud, MB; Sterle, HA; Carabajal, E; Tesan, F; Perazzo, JC; Cremaschi, GA; Rivera, ES; Medina, VA; Selective cytoprotective effect of histamine on doxorubicin-induced hepatic and cardiac toxicity in animal models. 1, 15059 (2015). WB, IHC, IP, Human, Mouse, Rat 27551485

Target Reference 1. Ng, S.-Y.; Gunning, P.; Eddy, R.; Ponte, P.; Leavitt, J.; Kedes, L.; Shows, T. : Chromosome 7 assignment of the human beta-actin functional gene (ACTB) and the chromosomal dispersion of pseudogenes. (Abstract) Cytogenet. Cell Genet. 40: 712 only, 1985.
2. Procaccio, V.; Salazar, G.; Ono, S.; Styers, M. L.; Gearing, M.; Davila, A.; Jimenez, R.; Juncos, J.; Gutekunst, C.-A.; Meroni, G.; Fontanella, B.; Sontag, E.; Sontag, J. M.; Faundez, V.; Wainer, B. H. : A mutation of beta-actin that alters depolymerization dynamics is associated with autosomal dominant developmental malformations, deafness, and dystonia. Am. J. Hum. Genet. 78: 947-960, 2006.
3. Ng, S.-Y.; Gunning, P.; Eddy, R.; Ponte, P.; Leavitt, J.; Shows, T.; Kedes, L. : Evolution of the functional human beta-actin gene and its multi-pseudogene family: conservation of the noncoding regions and chromosomal dispersion of pseudogenes. Molec. Cell. Biol. 5: 2720-2732, 1985.
Gene Symbol ACTB
Official Gene Full Name Actin, cytoplasmic 1
NCBI Gene Id 60
Protein Name Actin, cytoplasmic 1
Description of Target The primary site of action of cytochalasin B on cell motility processes is beta-actin. Habets et al. (1992) generated hybrids that harbor only specific regions of human chromosome 7 and assigned the ACTB locus to 7p15-p12. ACTB and the other assigned beta-actin-related sequences are dispersed over at least four different chromosomes including one locus assigned to the X chromosome. A mutation of beta-actin that alters depolymerization dynamics is associated with autosomal dominant developmental malformations, deafness, and dystonia.
Swissprot Id P60709
Protein Accession # NP_001092.1
Nucleotide Accession # NM_001101.3
Tissue Tool Find tissues and cell lines supported by DNA array analysis to express ACTB.
RNA Seq Find tissues and cell lines supported by RNA-seq analysis to express ACTB.
  1. What is the species homology for "ACTB Antibody (OAAD00224)"?

    The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human, Mouse, Rat".

  2. How long will it take to receive "ACTB Antibody (OAAD00224)"?

    This item is available "Domestic: within 1 week delivery | International: 1 week".

  3. What buffer format is "ACTB Antibody (OAAD00224)" provided in?

    This item is provided in "Liquid. In PBS Buffer.".
    Additional format options may be available. For more information please contact info@avivasysbio.com.

  4. What are other names for "ACTB Antibody (OAAD00224)"?

    This target may also be called "" in publications.

  5. What is the shipping cost for "ACTB Antibody (OAAD00224)"?

    The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.

  6. What is the guarantee for "ACTB Antibody (OAAD00224)"?

    All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.

  7. Can I get bulk pricing for "ACTB Antibody (OAAD00224)"?

    You can get bulk pricing for this item by going here.

  8. What is the molecular weight of the protein?

    The molecular weight reported by Uniprot for this item is "".
    Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics.

  9. What protocols are available for "ACTB Antibody (OAAD00224)"?

    We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data tab on the product page.

  10. What are positive controls for "ACTB"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  11. What are negative controls for "ACTB"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  12. What other proteins interact with "ACTB"?

    This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.

  13. What biological processes are associated with "ACTB"?

    This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.

  14. What cellular components are associated with "ACTB"?

    This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.

  15. What protein functions are associated with "ACTB"?

    This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.

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