ACTA1 Recombinant Antibody [25E3] (OACA12001)

Catalog No: OACA12001

Size:100UL

Western Blot
Positive WB detected in: Hela whole cell lysate, A549 whole cell lysate, Raw264.7 whole cell lysate, NIH/3T3 whole cell lysate, HepG2 whole cell lysate, Rat brain tissue, Rat heart tissue
All lanes: Actin antibody at 0.95ug/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 42 KDa
Observed band size: 42 KDa

Overlay histogram showing Hela cells stained with OACA12001 (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4℃. The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4℃. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.

Immunofluorescence staining of HepG2 cells with OACA12001 at 1:60,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).

IHC image of OACA12001 diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid
• Clonality: Recombinant Monoclonal
• Conjugation: Unconjugated
• Application: Enzyme-linked immunosorbent assay,
  Flow cytometry,
  Immunofluorescence,
  Immunohistochemistry,
  Western blot
• Reconstitution and Storage: -20°C or -80°C
• Immunogen: A synthesized peptide
• Purification: Affinity Purified

Target Info

• Gene Symbol: ACTA1
• Gene Full Name: actin alpha 1, skeletal muscle
• Alias Symbols: ACTA;actin, alpha skeletal muscle;Alpha-actin-1;ASMA;CFTD;CFTD1;CFTDM;MPFD;NEM1;NEM2;NEM3;nemaline myopathy type 3;SHPM.
• NCBI Gene Id: 58
• Protein Name: Actin, alpha skeletal muscle
• Description of Target: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
• Uniprot ID: P68133