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Datasheets/Manuals | Printable datasheet for anti-Act57B (OAAD00115) antibody |
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Predicted Species Reactivity | Human, Mouse, Rat, Chicken |
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Clonality | Monoclonal |
Clone | AC-40 |
Isotype | IgG2a |
Host | Mouse |
Application | WB, IHC, ICC |
Reconstitution and Storage | At -20C for one year. After reconstitution, at 4C for one month. It can also be aliquotted and stored frozen at -20C for longer time. |
Immunogen | Synthetic actin C-terminal peptide Ser-Gly-Pro-Ser-Ile-Val-His-Arg-Lys-Cys-Phe, attached to a Multiple Antigen Peptide (MAP) backbone. |
Purification | Purified by the goat anti-mouse IgG Affinity chromatography. |
Specificity | Human, mouse, rat, chicken. No cross reactivity with other proteins. |
Formulation | Lyophilized from 1.2% sodium acetate, with 2mg BSA and 0.01mg NaN3 as preservative. |
Application Info | Western blot At 2ug/ml with the appropriate system to detect Actin in cells and tissues. Immunohistochemistry(F) At 4ug/ml to detect Actin in formalin or acetone fixed tissues. Immunocytochemistry Suitable Other applications have not been tested. Optimal dilutions should be determined by end user. |
Storage Buffer | 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100ug/ml. |
Reference | 1.Gunning, P., Ponte, P., Okayama, H., Engel, J., Blau, H. and Kedes, L.Isolation and characterization of full-length cDNA clones for human alpha-, beta-, and gamma-actin mRNAs: skeletal but not cytoplasmic actins have an amino-terminal cysteine that is subsequently removed. Mol. Cell. Biol.1983; 3 (5), 787-795. 2.Goebel, H.H., Brockmann, K., Bonnemann, C.G., Warlo, I.A., Hanefeld, F., Labeit, S., Durling, H.J. and Laing, N.G.Actin-related myopathy without any missense mutation in the ACTA1 Gene. J. Child Neurol.2004; 19 (2), 149-153. 3.Laing, N.G., Clarke, N.F., Dye, D.E., Liyanage, K., Walker, K.R., Kobayashi, Y., Shimakawa, S., Hagiwara, T., Ouvrier, R., Sparrow, J.C., Nishino, I., North, K.N. and Nonaka, I.Actin mutations are one cause of congenital fibre type disproportion.Ann. Neurol.2004; 56 (5), 689-694 . |
Gene Symbol | Act57B |
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Gene Full Name | Actin-57B |
Alias Symbols | act 57A, ACT3, Act57, act57A, Act57A, act57b, act57B, Act57b, actin, Actin, actin-57B, anon-EST:CL2c12, ArpE, BEST:RE38067, C, CG10067, CG30294, chr2R:16452771..16452966, Dmel\CG10067, M, T10 |
NCBI Gene Id | 37368 |
Protein Name | Actin-57B |
Description of Target | Actin, a highly conserved protein, is a major component of both the cytoskeletal and contractile structures in the cell types. It varies in amount, being related to the type of differentiation and to the functional state of cells and tissues. The actins exhibit over 90% sequence homology, but each isoform has a unique NH2-terminal sequence. The isoforms are comprised of three alpha-actin, one beta-actin, two gamma-actin. Because the amino acid sequence of the C-terminal is the same for almost all actins, this antibody has been raised using a synthetic peptide corresponding to the C-terminal 11 residues. |
Uniprot ID | P53501 |
Protein Accession # | NP_523800.1 |
Nucleotide Accession # | NM_079076.4 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "Act57B Antibody (OAAD00115)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human, Mouse, Rat, Chicken".
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How long will it take to receive "Act57B Antibody (OAAD00115)"?
This item is available "Domestic: within 1-2 week delivery | International: within 1-2 week delivery".
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What buffer format is "Act57B Antibody (OAAD00115)" provided in?
This item is provided in "".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "Act57B Antibody (OAAD00115)"?
This target may also be called "act 57A, ACT3, Act57, act57A, Act57A, act57b, act57B, Act57b, actin, Actin, actin-57B, anon-EST:CL2c12, ArpE, BEST:RE38067, C, CG10067, CG30294, chr2R:16452771..16452966, Dmel\CG10067, M, T10" in publications.
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What is the shipping cost for "Act57B Antibody (OAAD00115)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "Act57B Antibody (OAAD00115)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "Act57B Antibody (OAAD00115)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "Act57B Antibody (OAAD00115)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "ACT57B"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "ACT57B"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "ACT57B"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "ACT57B"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "ACT57B"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "ACT57B"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.