Act57B Antibody (OAAD00115)

Catalog No: OAAD00115

Size:100 ug

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat, Chicken
• Clonality: Monoclonal
• Clone: AC-40
• Isotype: IgG2a
• Host: Mouse
• Application: WB, IHC, ICC
• Reconstitution and Storage: At -20C for one year. After reconstitution, at 4C for one month. It can also be aliquotted and stored frozen at -20C for longer time.
• Immunogen: Synthetic actin C-terminal peptide Ser-Gly-Pro-Ser-Ile-Val-His-Arg-Lys-Cys-Phe, attached to a Multiple Antigen Peptide (MAP) backbone.
• Purification: Purified by the goat anti-mouse IgG Affinity chromatography.
• Specificity: Human, mouse, rat, chicken.
  No cross reactivity with other proteins.
• Formulation: Lyophilized from 1.2% sodium acetate, with 2mg BSA and 0.01mg NaN3 as preservative.
• Application Info: Western blot At 2ug/ml with the appropriate system to detect Actin in cells and tissues.
  Immunohistochemistry(F) At 4ug/ml to detect Actin in formalin or acetone fixed tissues.
  Immunocytochemistry Suitable Other applications have not been tested.
  Optimal dilutions should be determined by end user.
• Storage Buffer: 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100ug/ml.
• Reference: 1.Gunning, P., Ponte, P., Okayama, H., Engel, J., Blau, H. and Kedes, L.Isolation and characterization of full-length cDNA clones for human alpha-, beta-, and gamma-actin mRNAs: skeletal but not cytoplasmic actins have an amino-terminal cysteine that is subsequently removed.
  Mol. Cell. Biol.1983; 3 (5), 787-795.
  2.Goebel, H.H., Brockmann, K., Bonnemann, C.G., Warlo, I.A., Hanefeld, F., Labeit, S., Durling, H.J. and Laing, N.G.Actin-related myopathy without any missense mutation in the ACTA1 Gene.
  J. Child Neurol.2004; 19 (2), 149-153.
  3.Laing, N.G., Clarke, N.F., Dye, D.E., Liyanage, K., Walker, K.R., Kobayashi, Y., Shimakawa, S., Hagiwara, T., Ouvrier, R., Sparrow, J.C., Nishino, I., North, K.N. and Nonaka, I.Actin mutations are one cause of congenital fibre type disproportion.Ann. Neurol.2004; 56 (5), 689-694 .

Target Info

• Gene Symbol: Act57B
• Gene Full Name: Actin-57B
• Alias Symbols: act 57A, ACT3, Act57, act57A, Act57A, act57b, act57B, Act57b, actin, Actin, actin-57B, anon-EST:CL2c12, ArpE, BEST:RE38067, C, CG10067, CG30294, chr2R:16452771..16452966, Dmel\CG10067, M, T10
• NCBI Gene Id: 37368
• Protein Name: Actin-57B
• Description of Target: Actin, a highly conserved protein, is a major component of both the cytoskeletal and contractile structures in the cell types. It varies in amount, being related to the type of differentiation and to the functional state of cells and tissues. The actins exhibit over 90% sequence homology, but each isoform has a unique NH2-terminal sequence. The isoforms are comprised of three alpha-actin, one beta-actin, two gamma-actin. Because the amino acid sequence of the C-terminal is the same for almost all actins, this antibody has been raised using a synthetic peptide corresponding to the C-terminal 11 residues.
• Uniprot ID: P53501
• Protein Accession #: NP_523800.1
• Nucleotide Accession #: NM_079076.4