Catalog No: OCOA14336
Size:20UG
Price: $179.00
SKU
OCOA14336
Availability: Domestic: within 1 week delivery | International: 1 week
Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.

ACSL1 Protein Lysate (OCOA14336)

Datasheets/ManualsPrintable datasheet for ACSL1 Protein Lysate (OCOA14336)
Product Info
Predicted Species ReactivityHomo sapiens
Product FormatLyophilized
HostHEK293T
Additional InformationComponents: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
::Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution and StorageThe lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
TagC-MYC/DDK
DescriptionACSL1 HEK293T cell transient overexpression lysate (as WB positive control)
Gene SymbolACSL1
Gene Full Nameacyl-CoA synthetase long chain family member 1
Alias SymbolsACS1;acyl-CoA synthetase 1;arachidonate--CoA ligase;FACL1;FACL2;fatty-acid-Coenzyme A ligase, long-chain 1;fatty-acid-Coenzyme A ligase, long-chain 2;LACS;LACS 1;LACS 2;LACS1;LACS2;lignoceroyl-CoA synthase;long-chain acyl-CoA synthetase 1;long-chain acyl-CoA synthetase 2;long-chain fatty acid-CoA ligase 2;long-chain fatty-acid-coenzyme A ligase 1;long-chain-fatty-acid--CoA ligase 1;palmitoyl-CoA ligase 1;palmitoyl-CoA ligase 2;paltimoyl-CoA ligase 1;phytanate--CoA ligase.
NCBI Gene Id2180
Protein NameA8K9T3, NM_001995, NP_001986, P33121
Description of TargetArachidonate--CoA ligase, Homo sapiens acyl-CoA synthetase long chain family member 1 (ACSL1), transcript variant 1, mRNA, Long-chain-fatty-acid--CoA ligase 1, long-chain-fatty-acid--CoA ligase 1 isoform a [Homo sapiens]
Uniprot IDhttps://www.uniprot.org/uniprot/P33121
Protein Accession #https://www.ncbi.nlm.nih.gov/protein/NP_001986
Nucleotide Accession #https://www.ncbi.nlm.nih.gov/nuccore/NM_001995
Molecular Weight77.8 kDa
Write Your Own Review
You're reviewing:ACSL1 Protein Lysate (OCOA14336)
Your Rating