Catalog No: OPPA00694 (Formerly GWB-257EF3)
Size:2UG
Price: $75.00
SKU
OPPA00694
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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- $55: Antibody & Protein in US
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Datasheets/Manuals | Printable datasheet for OPPA00694 |
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Predicted Species Reactivity | Mouse |
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Product Format | Sterile filtered and lyophilized from 0.5 mg/ml in PBS buffer. |
Host | HEK293 (Human Embryonic Kidney Cell Line) |
Application | ELISA, WB |
Additional Information | Solubility: Add 0.2 ml of deionized water and let the lyophilized pellet dissolve completely. |
:: | Biological Activity: Full-length mouse adiponectin activates AMP-activated protein kinase in hepatocyte and activates AMPK in HepG2 human hepatocytes at a concentration of 1 ug/ml.Mouse adiponectin mammalian cell derived inhibits glucose production as shown by in-vitro gluconeogenesis assay in primary rat hepatocytes. |
:: | Product Introduction: Adiponectin is an important negative regulator in hematopoiesis and immune systems. It may be involved in ending inflammatory responses through its inhibitory functions. Inhibits endothelial nf-kappa-b signaling through a camp-dependent pathway. Inhibits tnf-alpha- induced expression of endothelial adhesion molecules. Involved in the control of fat metabolism and insulin sensitivity. Product Description: The Acrp30 Mouse Recombinant is fused with FLAG tag. |
Reconstitution and Storage | Store lyophilized APM-1 at -20C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4C for a limited period of time; it does not show any change after two weeks at 4C. The lyophilized protein remains stable until the expiry date when stored at -20C. |
Purity | Purity of adiponectin mouse recombinant is >98% (HPLC and SDS PAGE analyzed). |
Peptide Sequence | EDDVTTTEEL APALVPPPKG TCAGWMAGIP GHPGHNGTPG RDGRDGTPGE KGEKGDAGLL GPKGETGDVG MTGAEGPRGF PGTPGRKGEP GEAAYMYRSA FSVGLETRVT VPNVPIRFTK IFYNQQNHYD GSTGKFYCNI PGLYYFSYHI TVYMKDVKVS LFKKDKAVLF TYDQYQEKNV DQASGSVLLH LEVGDQVWLQ VYGDGDHNGL YADNVNDSTF TGFLLYHDTN DYKDDDDK. |
Source | HEK293 Cells |
Application Info | ELISA, Western Blot, Cell culture and/or animal studies |
Gene Symbol | mAcrp30 HEK |
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Alias Symbols | a, Ad, ap, APN, Acd, Acr, Acdc, Adid, GBP2, adip, apM1, 30kDa, GBP28, adipo, Acrp30 |
NCBI Gene Id | 11450 |
Protein Name | Adiponectin |
Description of Target | Recombinant Mouse Adiponectin glycosilated, HEK |
Uniprot ID | Q60994 |
Protein Accession # | NP_033735.3 |
Protein Size (# AA) | Recombinant |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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