Catalog No: OPPA00692 (Formerly GWB-FB0BD0)
Size:10UG
Price: $75.00
SKU
OPPA00692
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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- Phone: 858-552-6979
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- $55: Antibody & Protein in US
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Datasheets/Manuals | Printable datasheet for OPPA00692 |
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Predicted Species Reactivity | Human |
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Product Format | Acrp30 Human was filtered (0.4 um) and lyophilized from 0.5 mg/ml in 0.02M Tris buffer pH7.5, 0.15M NaCl. |
Host | E. Coli |
Application | WB |
Additional Information | Solubility: It is recommended to add deionized water to prepare a working stock solution of approximately 0.5mg/ml and let the lyophilized pellet dissolve completely. Product is not sterile |
:: | Quality Control Test: BCA - to determine content of the protein. SDS PAGE - to determine purity of the protein. |
:: | Product Introduction: Adiponectin, also referred to as Acrp30, AdipoQ and GBP-28, is a recently discovered 244 amino acid protein, the product of the apM1 gene, which is physiologically active and specifically and highly expressed in adipose cells (Adipokine). The protein belongs to the soluble defense collagen super family; it has a collagen-like domain structurally homologous with collagen VIII and X and complement factor C1q-like globular domain. APM-1 forms homotrimers, which are the building blocks for higher order complexes found circulating in serum. Product Description: The Acrp30 Human is created as a recombinant protein with N-terminal fusion of His Tag. The Adiponectin His-Tagged Fusion Protein, produced in E. coli, is 26.4 kDa protein containing 230 amino acid residues of the Acrp30 Human and 12 additional amino acid residues - HisTag (underlined). MRGSHHHHHHGSGHDQETTT QGPGVLLPLP KGACTGWMAG IPGHPGHNGA PGRDGRDGTP GEKGEKGDPG LIGPKGDIGE TGVPGAEGPR GFPGIQGRKG EPGEGAYVYR SAFSVGLETY VTIPNMPIRF TKIFYNQQNH YDGSTGKFHC NIPGLYYFAY HITVYMKDVK VSLFKKDKAM LFTYDQYQEN NVDQASGSVL LHLEVGDQVW LQVYGEGERN GLYADNDNDS TFTGFLLYHD TN. |
Reconstitution and Storage | Store lyophilized Acrp30 Human at -20C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted Acrp30 Human can be stored at 4C for a limited period of time; it does not show any change after two weeks at 4C. The lyophilized Acrp30 Human remains stable until the expiry date when stored at -20C. |
Purity | Acrp30 Human purity is greater than 95% as determined by SDS-PAGE. |
Gene Symbol | Acrp30 His |
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Alias Symbols | ACDC, ADPN, APM1, APM-1, GBP28, ACRP30, ADIPQTL1 |
NCBI Gene Id | 9370 |
Protein Name | Adiponectin |
Description of Target | Recombinant Human Adiponectin, His tag |
Uniprot ID | Q15848 |
Protein Accession # | NP_001171271.1 |
Protein Size (# AA) | Recombinant |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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