ACPP ELISA Kit (Human) (OKCD06095)

Catalog No: OKCD06095

Size:96WELLS

ACPP ELISA Kit (Human) (OKCD06095) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Prostatic Acid Phosphatase (PAP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Prostatic Acid Phosphatase (PAP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Prostatic Acid Phosphatase (PAP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Prostatic Acid Phosphatase (PAP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Prostatic Acid Phosphatase (PAP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Prostatic Acid Phosphatase (PAP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • ACPP Microplate: 96 Wells (12 x 8 Well strips)
  • ACPP Lyophilized Standard: 2
  • 100X ACPP HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Sensitivity: < 0.054ng/mL

Target Info

• Gene Symbol: ACP3
• Gene Full Name: acid phosphatase 3
• Alias Symbols: 5'-NT;5'-nucleotidase;acid phosphatase, prostate;ACP-3;ACPP;ecto-5'-nucleotidase;prostatic acid phosphatase;prostatic acid phosphotase;protein tyrosine phosphatase ACP3;thiamine monophosphatase;TM-PAP;TMPase.
• NCBI Gene Id: 55
• Protein Name: Prostatic acid phosphatase
• Description of Target: A non-specific tyrosine phosphatase that dephosphorylates a diverse number of substrates under acidic conditions (pH 4-6) including alkyl, aryl, and acyl orthophosphate monoesters and phosphorylated proteins. Has lipid phosphatase activity and inactivates lysophosphatidic acid in seminal plasma.
• Uniprot ID: P15309
• Protein Accession #: NP_001090.2
• Nucleotide Accession #: NM_001099.4