ACP5 ELISA Kit (Mouse) (OKCD06977)

Catalog No: OKCD06977

Size:96WELLS

ACP5 ELISA Kit (Mouse) (OKCD06977) Standard Curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Acid Phosphatase 5, Tartrate Resistant (ACP5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Acid Phosphatase 5, Tartrate Resistant (ACP5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Acid Phosphatase 5, Tartrate Resistant (ACP5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Acid Phosphatase 5, Tartrate Resistant (ACP5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 78-5,000pg/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Acid Phosphatase 5, Tartrate Resistant (ACP5) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Acid Phosphatase 5, Tartrate Resistant (ACP5) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • ACP5 Microplate: 96 Wells (12 x 8 Well strips)
  • ACP5 Lyophilized Standard: 2
  • 100X ACP5 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: < 32pg/mL

Target Info

• Gene Symbol: Acp5
• Gene Full Name: acid phosphatase 5, tartrate resistant
• Alias Symbols: acid phosphatase type V;T;tartrate-resistant acid ATPase;tartrate-resistant acid phosphatase type 5;TR;TRACP;TRAP;TR-AP;trATPase;type 5 acid phosphatase.
• NCBI Gene Id: 11433
• Protein Name: Tartrate-resistant acid phosphatase type 5
• Description of Target: May play a role in the process of bone resorption. The osteoclastic trap acts on nucleotide tri- and diphosphates with higher affinity, compared with other substrates.
• Uniprot ID: Q05117
• Protein Accession #: NP_001095874.1
• Nucleotide Accession #: NM_001102404.1