ACOX3 Antibody (OAGA01108)

Catalog No: OAGA01108

Size:100 ul

Sample (30 ug of whole cell lysate)
A: A549
7.5% SDS PAGE
OAGA01108 diluted at 1:1000

ACOX3 antibody detects ACOX3 protein by Western blot analysis.
A. 50 ug mouse liver lysate/extract
7.5 % SDS-PAGE
ACOX3 antibody (OAGA01108) dilution: 1:500

Immunohistochemical analysis of paraffin-embedded H441 xenograft, using ACOX3(OAGA01108) antibody at 1:500 dilution.

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: IHC-FFPE, WB
• Additional Information: Background: Acyl-Coenzyme A oxidase 3 also know as pristanoyl -CoA oxidase (ACOX3)is involved in the desaturation of 2-methyl branched fatty acids in peroxisomes. Unlike the rat homolog, the human gene is expressed in very low amounts in liver such that its mRNA was undetectable by routine Northern-blot analysis or its product by immunoblotting or by enzyme activity measurements. However the human cDNA encoding a 700 amino acid protein with a peroxisomal targeting C-terminal tripeptide S-K-L was isolated and is thought to be expressed under special conditions such as specific developmental stages or in a tissue specific manner in tissues that have not yet been examined. [provided by RefSeq]
  Positive Controls: A549, mouse liver
  Cellular Localization: Peroxisome
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant protein fragment contain a sequence corresponding to a region within amino acids 408 and 603 of ACOX3
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 1 mg/ml
• Application Info: Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: ACOX3
• Gene Full Name: Peroxisomal acyl-coenzyme A oxidase 3
• Alias Symbols: ACOX3 antibody, pristanoyl-CoA oxidase antibody, peroxisomal acyl-coenzyme A oxidase 3 antibody, acyl-Coenzyme A oxidase 3, pristanoyl antibody, branched-chain acyl-CoA oxidase antibody, BRCACox antibody, acyl-CoA oxidase 3, pristanoyl antibody
• NCBI Gene Id: 8310
• Protein Name: Peroxisomal acyl-coenzyme A oxidase 3
• Uniprot ID: O15254
• Protein Accession #: NP_001095137.1
• Molecular Weight: 70 kDa