Search Antibody, Protein, and ELISA Kit Solutions

ACOT12 Protein Lysate (OCOA06713)

20 ug
$129.00
In Stock
Request Bulk Order Quote

Predicted Species Reactivity:
Human
Product Format:
Lyophilized
Host:
HEK293T
Additional Information:
Components: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
:::
Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution and Storage:
The lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
Tag:
C-MYC/DDK
Datasheets/Manuals:
Printable datasheet for OCOA06713
Gene Symbol:
ACOT12
Alias Symbols:
ACOT12, Cach, CACH-1, STARD15, THEAL
NCBI Gene Id:
134526
Protein Accession #:
NP_570123
Nucleotide Accession #:
NM_130767
Molecular Weight:
61.9 kDa
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express ACOT12.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express ACOT12.

Product Reviews

Protocol:
Tips Information:

Tell us what you think about this item!

Write A Review
    Please, wait...