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Catalog No: OCOA06713
Size:20UG
Price: $179.00
SKU
OCOA06713
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ACOT12 Protein Lysate (OCOA06713)

Datasheets/ManualsPrintable datasheet for ACOT12 Protein Lysate (OCOA06713)
Product Info
Predicted Species ReactivityHomo sapiens
Product FormatLyophilized
HostHEK293T
Additional InformationComponents: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
::Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution and StorageThe lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
TagC-MYC/DDK
DescriptionACOT12 HEK293T cell transient overexpression lysate (as WB positive control)
Gene SymbolACOT12
Gene Full Nameacyl-CoA thioesterase 12
Alias Symbolsacetyl-coenzyme A thioesterase;acyl-CoA thioester hydrolase 12;acyl-coenzyme A thioesterase 12;Cach;CACH-1;cytoplasmic acetyl-CoA hydrolase 1;cytosolic acetyl-CoA hydrolase;hCACH-1;STARD15;StAR-related lipid transfer (START) domain containing 15;START domain-containing protein 15;THEAL.
NCBI Gene Id134526
Protein NameNM_130767, NP_570123, Q8WYK0
Description of TargetAcyl-coenzyme A thioesterase 12, acyl-coenzyme A thioesterase 12 [Homo sapiens], Homo sapiens acyl-CoA thioesterase 12 (ACOT12), mRNA
Uniprot IDhttps://www.uniprot.org/uniprot/Q8WYK0
Protein Accession #https://www.ncbi.nlm.nih.gov/protein/NP_570123
Nucleotide Accession #https://www.ncbi.nlm.nih.gov/nuccore/NM_130767
Molecular Weight61.9 kDa
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