ACLY ELISA Kit (Bovine) (OKEH08002)

Catalog No: OKEH08002

Size:96T

Product Info

• Predicted Species Reactivity: Bos taurus, Bovine
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology ACLY ELISA Kit (Bovine) (OKEH08002) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ACLY has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ACLY is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ACLY captured in well.
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 89% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=8.3% (n = 20)
  Mean Inter-assay CV%: <=11.8% (n = 20)
• ELISA Kit Component:
  • ACLY Microplate: 96 Wells (12 x 8 Well strips)
  • ACLY Lyophilized Standard: 2
  • 100X Biotinylated ACLY Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: ATP-citrate synthase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. Has a central role in de novo lipid synthesis. In nervous tissue it may be involved in the biosynthesis of acetylcholine.
  Subcellular Location: Cytoplasm
  Unigene: Bt.41387
  String: 9913.ENSBTAP00000022258
  KEGG: bta:511135
  Pfam: PF16114
• Reconstitution and Storage: Store as indicated in product manual.
• Specificity: Natural and recombinant Bovine ATP-citrate synthase
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: ACLY
• Gene Full Name: ATP citrate lyase
• Alias Symbols: ATP-citrate (pro-S-)-lyase;ATP-citrate synthase;citrate cleavage enzyme.
• NCBI Gene Id: 511135
• Protein Name: Q32PF2
• Description of Target: ATP-citrate synthase
• Uniprot ID: https://www.uniprot.org/uniprot/Q32PF2
• Protein Accession #: NP_001032534.1
• Nucleotide Accession #: NM_001037457.1