ACLY Antibody - N-terminal region (OAGA04026)

Catalog No: OAGA04026

Size:100 ul

Sample (30 ug of whole cell lysate)
A: 293T
B: A431
C: Jurkat
D: Raji
5% SDS PAGE
diluted at 1:5000

Immunohistochemical analysis of paraffin-embedded Hepatoma, using ATP citrate lyase antibody at 1:500 dilution.

ATP citrate lyase antibody [N1N2], N-term detects ACLY protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: ACLY protein stained by ATP citrate lyase antibody [N1N2], N-term diluted at 1:500.
Blue: Hoechst 33343 staining.

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Liquid 1X PBS, 1% BSA, 20% glycerol (pH 7) with 0.01% Thimerosal added as a preservative
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: WB, ICC, IF, IHC-P
• Reconstitution and Storage: Keep as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze/thaw cycles.
• Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human ATP citrate lyase. The exact sequence is proprietary.
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 1 mg/ml
• Application Info: WB: 1:1000~10000
  ICC/IF: 1:100~1000
  IHC-P: 1:100~1000
• Publications:

  ATP-citrate lyase promotes axonal transport across species. Nat Commun. 12, 5878 (2021)

Target Info

• Gene Symbol: ACLY
• Gene Full Name: ATP citrate lyase
• Alias Symbols: ACL, ATPCL, CLATP
• NCBI Gene Id: 47
• Protein Name: ATP-citrate synthase
• Description of Target: ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Multiple transcript variants encoding distinct isoforms have been identified for this gene.
• Uniprot ID: P53396
• Protein Accession #: NP_001087.2
• Nucleotide Accession #: NM_001096.2
• Molecular Weight: 121 kDa