ACLY Antibody - C-terminal region (OAGA04024)

Catalog No: OAGA04024

Size:100 ul

Sample (50 ug of whole cell lysate)
A: mouse brain
5% SDS PAGE
diluted at 1:2000
The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

ATP citrate lyase antibody [C2C3], C-term detects ACLY protein by western blot analysis.
A. 30 ug PC-12 whole cell lysate/extract
B. 30 ug Rat2 whole cell lysate/extract
5% SDS-PAGE
ATP citrate lyase antibody [C2C3], C-term dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Sample (30 ug of whole cell lysate)
A: HeLa
B: Raji
7.5% SDS PAGE
diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

ATP citrate lyase antibody [C2C3], C-term detects ATP citrate lyase protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: ATP citrate lyase protein stained by ATP citrate lyase antibody [C2C3], C-term diluted at 1:200.
Blue: Hoechst 33342 staining.

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Liquid 0.1M Tris, 0.1M Glycine, 10% glycerol (pH 7) with 0.01% Thimerosal as a preservative
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: ICC, IF, WB
• Reconstitution and Storage: Keep as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze/thaw cycles.
• Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human ATP citrate lyase. The exact sequence is proprietary.
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 1 mg/ml
• Application Info: WB: 1:500~3000
  ICC/IF: 1:100~1000

Target Info

• Gene Symbol: ACLY
• Gene Full Name: ATP citrate lyase
• Alias Symbols: ACL, ATPCL, CLATP
• NCBI Gene Id: 47
• Protein Name: ATP-citrate synthase
• Description of Target: ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Multiple transcript variants encoding distinct isoforms have been identified for this gene.
• Uniprot ID: P53396
• Protein Accession #: NP_001087.2
• Nucleotide Accession #: NM_001096.2
• Molecular Weight: 121 kDa