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Catalog No: OKEH03313
Size:96T
Price: $525.00
SKU
OKEH03313
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
COA DatasheetPrintable COA datasheet for OKEH03313
Product Info
Predicted Species ReactivityRat, Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology ACHE ELISA Kit (Rat) (OKEH03313) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ache has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Ache is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Ache captured in well.
ELISA Kit Range0.625-40ng/mL
ELISA Kit Component
ComponentAmount
Anti-Ache Microplate96 Wells (12 x 8 Well strips)
Ache Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated Ache Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity0.312 ng/mL
SpecificityNatural and recombinant Rat Acetylcholinesterase
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolAche
Gene Full Nameacetylcholinesterase
Alias Symbolsacetylcholinesterase;glycolipid-anchored form of acetylcholinesterase.
NCBI Gene Id83817
Protein NameP37136
Description of TargetAcetylcholinesterase
Uniprot IDhttps://www.uniprot.org/uniprot/P37136
Protein Accession #NP_742006.1
Nucleotide Accession #NM_172009.1
Protein Size (# AA)614
Molecular Weight68 kDa
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